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  4. Rat Cyclooxygenase-1(COX-1) ELISA Kit

Rat Cyclooxygenase-1(COX-1) ELISA Kit

  • 中文名称：
    大鼠环加氧酶1(COX-1)酶联免疫试剂盒
  • 货号：
    CSB-E13416r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This rat cyclooxygenase-1 (COX-1) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of rat COX-1 in different samples, including serum, plasma, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of COX-1 bound in the initial step.

    COX-1 (PTGS1) is a constitutive enzyme expressed in the vascular endothelium, in platelets, and in many other cells in the body. It is responsible for the initial cyclooxygenase reaction, the target for NSAIDs, the conversion of arachidonic acid to PGG2, and the subsequent peroxidase reaction that converts PGG2 to PGH2. Several studies suggest that COX-1 also plays a role in neuroinflammation. COX-1 also mediates prostaglandin-dependent gastric protection. In gastric mucosa, COX-1 is involved in the synthesis of PGE2 and PGI2, which exert cytoprotective effects on several aspects of gastric function such as an increase of bicarbonate and mucus secretion, reduction of gastric acid and pepsin secretion, and maintaining adequate blood flow to the mucosa.

  • 别名：
    Ptgs1 ELISA Kit; Cox-1 ELISA Kit; Cox1 ELISA Kit; Prostaglandin G/H synthase 1 ELISA Kit; EC 1.14.99.1 ELISA Kit; Cyclooxygenase-1 ELISA Kit; COX-1 ELISA Kit; Prostaglandin H2 synthase 1 ELISA Kit; PGH synthase 1 ELISA Kit; PGHS-1 ELISA Kit; PHS 1 ELISA Kit; Prostaglandin-endoperoxide synthase 1 ELISA Kit
  • 缩写：
    PTGS1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 pg/mL-500 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat COX-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %86
    Range %80-92
    1:200Average %92
    Range %88-97
    1:400Average %93
    Range %85-99
    1:800Average %101
    Range %95-107
  • 回收率：
    The recovery of rat COX-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)9084-94
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5002.448 2.352 2.400 2.262
    2502.035 1.989 2.012 1.874
    1251.512 1.501 1.507 1.369
    62.50.922 0.879 0.901 0.763
    31.20.491 0.473 0.482 0.344
    15.60.336 0.318 0.327 0.189
    7.80.207 0.199 0.203 0.065
    00.139 0.137 0.138 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Dual cyclooxygenase and peroxidase in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate, with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates arachidonate (AA, C20:4(n-6)) to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide PGH2, the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells.
  • 亚细胞定位：
    Microsome membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein.
  • 蛋白家族：
    Prostaglandin G/H synthase family
  • 数据库链接：