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Rat Cytochrome c(CYCS) ELISA kit

  • 中文名称：
    大鼠细胞色素C(CYCS)酶联免疫试剂盒
  • 货号：
    CSB-EL006328RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat CYCS ELISA Kit was designed for the quantitative measurement of Rat CYCS protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

  • 别名：
    CycsCytochrome c ELISA Kit; somatic ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CYCS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %90
    Range %87-94
    1:2Average %89
    Range %85-96
    1:4Average %92
    Range %90-95
    1:8Average %91
    Range %87-96
  • 回收率：
    The recovery of rat CYCS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-96
    EDTA plasma (n=4)9084-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    50002.581 2.458 2.520 2.427
    25002.234 2.186 2.210 2.117
    12501.638 1.621 1.630 1.537
    6251.103 1.097 1.100 1.007
    3120.692 0.672 0.682 0.589
    1560.393 0.386 0.390 0.297
    780.236 0.223 0.230 0.137
    00.094 0.092 0.093 　
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.; Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
  • 亚细胞定位：
    Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane.
  • 蛋白家族：
    Cytochrome c family
  • 组织特异性：
    Found in embryos and in adult liver and heart.
  • 数据库链接：