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  4. Rat Follistatin,FS ELISA Kit

Rat Follistatin,FS ELISA Kit

  • 中文名称：
    大鼠卵泡抑素(FS)酶联免疫试剂盒
  • 货号：
    CSB-E08507r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat FST ELISA Kit was designed for the quantitative measurement of Rat FST protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.2 ng/mL-40 ng/mL and the sensitivity is 0.1 ng/mL.

  • 别名：
    FstFollistatin ELISA Kit; FS ELISA Kit; Activin-binding protein ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.2 ng/mL-40 ng/mL
  • 灵敏度：
    0.1 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<15%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat FS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %84
    Range %81-89
    1:2Average %95
    Range %92-98
    1:4Average %102
    Range %98-106
    1:8Average %89
    Range %84-93
  • 回收率：
    The recovery of rat FS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9389-95
    EDTA plasma (n=4)8985-93
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2Average
    400.167 0.160 0.164
    100.401 0.432 0.417
    3.20.549 0.536 0.543
    0.80.951 0.942 0.947
    0.21.485 1.478 1.482
    02.548 2.409 2.479
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH).
  • 基因功能参考文献：
    1. These data suggest that ghrelin inhibits myocardial fibrosis, attenuates left ventricular remodeling, and eventually improves cardiac function by adjusting Act A/FS imbalance. PMID: 29113826
    2. manifestation of polycystic ovarian syndrome-like phenotype following prenatal exposure to excess androgen is associated with irregularity in expression of the FST gene during the estrus cycle PMID: 28277126
    3. Results show that Myostatin and follistatin protein expression is reduced in the long-term hypertrophied myocardium from spontaneously hypertensive rats with heart failure. PMID: 27128567
    4. Myogenin, myostatin, and follistatin expression is lower and myogenic regulatory factor (MRF)4 levels higher in spontaneously hypertensive rats (SHR) with heart failure compared to the Wistar-Kyoto strain. PMID: 22464481
    5. Expression of follistatin and myostatin mRNA is increased in muscle and subcutaneous fat of sedentary diabetic rats compared with sedentary control rats. PMID: 22332899
    6. The ratio of follistatin/myostatin could be used to monitor the progress of target muscle atrophy and recovery. PMID: 22033906
    7. Fst expression is necessary for the proliferation of small hepatocytes. PMID: 21826650
    8. Effects of training and androgens on MYOPRO and FOLLI concentrations in blood and skeletal muscle, were investigated. PMID: 20801187
    9. During chronic heart failure, decreased skeletal muscle trophism is combined with unchanged myostatin and reduced follistatin expression. PMID: 20002838
    10. demonstrate that levels of follistatin mRNA show two surges during the estrous cycle; a relationship between ovarian follistatin expression and progesterone secretion is also suggested PMID: 11906933
    11. PACAP and GnRH down-regulate follicle-stimulating hormone-beta mRNA levels by stimulating follistatin gene expression. PACAP influences FSH production selectively by a paracrine mechanism through folliculostellate cells that involves follistatin. PMID: 12088867
    12. frequency regulation of FSHbeta transcription involves both direct actions of GnRH and indirect effects, via changes in pituitary FS expression PMID: 12193535
    13. we studied the expression of follistatin in rat and mouse liver tumors as a possible mechanism to overcome activin growth control, 40% of the tumors displayed increased levels of follistatin mRNA when compared to tumor-surrounding liver tissue. PMID: 12203361
    14. follistatin originating from anterior pituitary folliculostellate cells participates in mediating the effects of IL-1beta, glucocorticoids, and other agents on the response of pituitary cells to activins. PMID: 12538636
    15. crystal structure of the presumed heparan sulfate-binding domain of follistatin, both alone and in complex with the small heparin analogs sucrose octasulfate and D-myo-inositol hexasulfate PMID: 12867435
    16. inhibin is a major regulator of FS, both by transcriptional and nontranscriptional mechanisms. PMID: 14561646
    17. Data suggest that betaA- and betaC-activin subunit proteins and follistatin are autocrine growth regulators in regenerating liver and when expressed independently lead to hepatocyte apoptosis or mitosis in a subset of hepatocytes. PMID: 15821113
    18. The roles of activin A and follistatin in hepatic fribrogenesis are reported. PMID: 16123203
    19. Follistatin was overexpressed while both activin subunits were downregulated in the majority of rat and human liver tumours PMID: 16935389
    20. Follistatin mRNA levels increased significantly with GnRH treatment in young animals but were not significantly changed in the middle aged females. PMID: 17344471
    21. intron 1 of the follistatin gene has a critical role in mediating Smad-dependent effects of activin and regulating the expression level of this gene PMID: 18184649
    22. Activin betaA subunit, follistatin and follistatin-like 3 are expressed in the endometrium of ovariectomized rats and regulated by estrogen replacement PMID: 18781389
    23. Fst expression in the pineal gland exhibits a ~20-fold increase at night, reaching levels that are > 16 fold higher than in other tissues. Expression is controlled by an adrenergic-cyclic AMP mechanism. PMID: 19103603

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  • 亚细胞定位：
    Secreted.
  • 数据库链接：