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Rat Gastrin ELISA Kit

  • 中文名称：
    大鼠胃泌素(Gastrin)酶联免疫试剂盒
  • 货号：
    CSB-E12743r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This rat gastrin (GAST) ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of rat gastrin in multiple samples, including serum, plasma, cell culture supernates, or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of gastrin bound in the initial step.

    Gastrin is a peptide hormone that is produced in the G cells of the gastric antrum and duodenum and is then released into the blood circulation. Gastrin exerts its actions by binding to the CCK-B on the parietal and enterochromaffin cells of the gastric body. It is trophic for normal gastrointestinal epithelium. It primarily contributes to increasing gastric mucosal growth, gastric motility, and secretion of hydrochloric acid (HCl) into the stomach. Studies have shown that gastrin is involved in the stimulation of growth of several gastrointestinal cancers including gastric cancer.

  • 别名：
    Gast ELISA Kit; Gas ELISA Kit; Gastrin [Cleaved into: Big gastrin ELISA Kit; Gastrin-34 ELISA Kit; G34); Gastrin] ELISA Kit
  • 缩写：
    GAST
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates ,tissue homogenates
  • 检测范围：
    0.312 pg/mL-20 pg/mL
  • 灵敏度：
    0.078 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat gastrin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %90
    Range %85-95
    1:200Average %94
    Range %90-98
    1:400Average %99
    Range %95-104
    1:800Average %102
    Range %98-107
  • 回收率：
    The recovery of rat gastrin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9288-96
    EDTA plasma (n=4)10095-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    202.496 2.537 2.517 2.390
    102.181 2.192 2.187 2.060
    51.795 1.741 1.768 1.641
    2.51.353 1.313 1.333 1.206
    1.250.878 0.898 0.888 0.761
    0.6250.369 0.366 0.368 0.241
    0.3120.242 0.259 0.251 0.124
    00.123 0.130 0.127 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
  • 基因功能参考文献：
    1. we examined the chronic effects of a GLP-1-gastrin dual agonist ZP3022 on glycemic control and beta cell dysfunction in overtly diabetic Zucker Diabetic Fatty PMID: 25849341
    2. treatment with gastrin, a CCK2R agonist, stimulated the secretion of GLP-1, and that this effect was likely due to increased expression of proglucagon and PCSK1 (also known as prohormone convertase 3 (PC3 gene)). PMID: 25601282
    3. The results suggested that clusterin may contribute to gastrin's growth-promoting effect on the oxyntic mucosa. PMID: 21995960
    4. plays a role in the development of gastric mucosa, ECL cells and A-like cells in newborn and young rats PMID: 12220729
    5. role in increasing expression of COX-2 mRNA and protein, the activity of the COX-2 promoter, and the release of prostaglandin E(2) PMID: 12239223
    6. High temperature can affect gastrin, somatostatin and motilin production in the gastric antral mucosa of rats with gastric ulcer. PMID: 12376287
    7. study demonstrates that gastrin enhances gastric mucosal integrity through cyclooxygenase-2 PMID: 12388195
    8. Data suggest that distention of isolated rat stomach stimulates somatostatin release via cholinergic and non-cholinergic TTX-insensitive pathways, and that these pathways are responsible for distention-induced inhibition of gastrin release. PMID: 12508367
    9. gastrin induces Akt through activation of CCKB receptors and of intracellular calcium-dependent, PKC-independent, pathways PMID: 15351698
    10. the role of JAK-dependent pathways in the regulation of E-cadherin-dependent cell-cell adhesion by gastrin PMID: 15530849
    11. gastrin- and PACAP-stimulated secretion of histamine and pancreastatin is inhibited by somatostatin, misoprostol and galanin in enterochromaffin-like cells PMID: 15935492
    12. hypogastrinemia and impaired enterochromaffin cell function in the oxyntic mucosa of the stomach might be partially responsible for the reduction in body weight that occurs after gastric bypass PMID: 16645284
    13. G cells are luminal sampling chemomechanosensory cells whose gastrin secretion is regulated by neural, hormonal, luminal, and mechanical factors through protein kinase A activation, cAMP signaling, and mitogen-activated protein kinase phosphorylation. PMID: 19208342

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Gastrin/cholecystokinin family
  • 数据库链接：

    KEGG: rno:25320

    STRING: 10116.ENSRNOP00000019863

    UniGene: Rn.9754