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  4. Rat Insulin-like growth factor 2,IGF-2 ELISA Kit

Rat Insulin-like growth factor 2,IGF-2 ELISA Kit

  • 中文名称：
    大鼠胰岛素样生长因子2(IGF-2)酶联免疫试剂盒
  • 货号：
    CSB-E04585r
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat IGF2 ELISA Kit was designed for the quantitative measurement of Rat IGF2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 78 pg/mL-5000 pg/mL and the sensitivity is 19.5 pg/mL.

  • 别名：
    Igf2 ELISA Kit; Igf-2 ELISA Kit; Insulin-like growth factor II ELISA Kit; IGF-II ELISA Kit; Multiplication-stimulating activity ELISA Kit; MSA ELISA Kit; Multiplication-stimulating polypeptide) [Cleaved into: Insulin-like growth factor II; Preptin] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    78 pg/mL-5000 pg/mL
  • 灵敏度：
    19.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IGF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:100Average %86
    Range %83-89
    1:200Average %99
    Range %85-104
    1:400Average %92
    Range %88-96
    1:800Average %87
    Range %85-89
  • 回收率：
    The recovery of rat IGF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 105101-109
    EDTA plasma (n=4)9794-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    50002.402 2.459 2.431 2.329
    25002.139 2.145 2.142 2.040
    12501.674 1.662 1.668 1.566
    6251.085 1.098 1.092 0.990
    3120.538 0.548 0.543 0.441
    1560.314 0.326 0.320 0.218
    780.196 0.186 0.191 0.089
    00.101 0.103 0.102
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF2 is influenced by placental lactogen. Also involved in tissue differentiation. In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver. Acts as a ligand for integrin which is required for IGF2 signaling. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation. Inhibits myoblast differentiation and modulates metabolism via increasing the mitochondrial respiration rate.; Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
  • 基因功能参考文献：
    1. We aimed to investigate whether TSN protected H9c2 cardiomyocytes from AngII-induced activation of IGF-2R pathway (IGF-2 and IGF-2R )and hypertrophy by mediating through Estrogen receptors PMID: 28812967
    2. Post-weaning high-fat (HF) diet intake resulted in the reduction of small follicles in adulthood, whereas maternal HF diet had long-term deleterious consequences on female offspring steroidogenesis and coincided with alteration of the upregulation of the imprinted gene Igf2 and changes in ovarian circadian rhythms. PMID: 28417351
    3. The results showed that f1 offspring exposed to p,p'-DDE exhibited impaired glucose tolerance, abnormal insulin secretion, beta-cell dysfunction and altered Igf2 and H19 expression induced by Igf2/H19 hypomethylation, which could be transferred to the F3 offspring through the male germ line. PMID: 28867213
    4. The IGF2 was further de-regulated to assess its mechanism in miR-193-5p-downreuglation induced angiogenic regulation. PMID: 28735866
    5. The hypomethylation observed at the H19 DMR corroborates with aberrant expression of Igf2 and H19 imprinted genes in the resorbed embryos sired by ERb agonist-treated males PMID: 28362134
    6. IGF2 expression in the rat brain both in basal condition and following learning predominantly derives from the maternal allele. PMID: 26495851
    7. Ancestral TCDD exposure may promote epigenetic transgenerational alterations of imprinted gene Igf2 in adult somatic tissue. PMID: 26455773
    8. This study suggests that bisphenol A exposure during early life can result in generational transmission of glucose intolerance and beta-cell dysfunction in the offspring through male germ line, which is associated with hypermethylation of Igf2 in islets. PMID: 26276081
    9. Allele-specific and total expression of hippocampal Igf2 is affected by maternal, grandmaternal caloric restriction in a strain and sex-specific manner. PMID: 24845189
    10. IGF-II may therefore contribute to the mechanisms of neuroprotection. PMID: 24667322
    11. We found that paternal folate deficiency induced a decrease in fetal liver folate content, IGF-2 expression, and the percentage of 5-mC in the fetal whole brain. PMID: 23229416
    12. IGF-IR-mediated activation of intracellular signaling may play a critical role during oxidative stress-induced apoptosis in necrotizing enterocolitis. PMID: 22434232
    13. IGF2 may play a role in tooth differentiation. PMID: 21959847
    14. IMP3 as a glioblastoma-specific proproliferative and proinvasive marker acting through IGF-2 resulting in the activation of oncogenic PI3K and MAPK pathways PMID: 21613208
    15. maternal exposure to a low protein diet and folic acid during gestation alters gene expression of Igf2 and H19 in the liver by regulating the DNA methylation of these genes PMID: 20671425
    16. Inhibitory avoidance learning leads to an increase in hippocampal expression of IGF-II, which requires the transcription factor CCAAT enhancer binding protein beta and is essential for memory consolidation PMID: 21270887
    17. Results indicate that undernutrition alters the timing and intensity of neonatal beta-cell ontogeny, and suggest that pancreatic IGF-2 in undernourished rats may contribute to the partial suppression of the developmental wave of beta-cell apoptosis. PMID: 19648158
    18. Our results show that maternal malnutrition ameliorates beta-cell mass in undernourished goto-kakizaki fetuses and a specific pancreatic IGF-2 increase may be instrumental in this effect. PMID: 19919529
    19. Data provide evidence for the role for estrogen in acquisition of imprint at Igf2/H19 DMR in testis and help in understanding molecular mechanism of environmental estrogens impacting male fertility. PMID: 19683557
    20. Expression of IGF-II in early experimental hepatocellular carcinomas PMID: 12532445
    21. Igf2 protectes theca-interstitial cell cells from apoptosis, reducing DNA fragmentation. PMID: 15833775
    22. IGF-2 expression in amoeboid microglial cells in the developing brain is related to their activation. PMID: 16448778
    23. The increased cardiac IGF-II, IGF-IIR, caspase 9, and cellular apoptosis were also found in hypertensive rats with abdominal aorta ligation. PMID: 16825605
    24. Hepatocellular carcinomas of the albumin SV40 T-antigen transgenic rat display fetal-like re-expression of Igf2. PMID: 17393425
    25. IGF2 and IGF1R have roles in beta cell mass anomaly in embryonic pancreas PMID: 17476475
    26. The insulin-like growth factor II expression was found to be predominant in hepatocellular carcinoma and in early preneoplastic lesions. PMID: 17554210
    27. Presence of IGF-II variants, including proIGF-II, IGF-II(1-87), and mature IGF-II, in the rat circulation during postnatal life, challenging current impression that IGF-II is absent from sera of adult rats. PMID: 17628003
    28. Gestational choline deficiency causes global and Igf2 gene DNA hypermethylation by up-regulation of Dnmt1 expression. PMID: 17724018
    29. PCR analysis of Igf2 gene by using of microsatellite D1Mgh22 has shown polymorphism between hereditary hypertriglyceridemic and Lewis rats. PMID: 18418699
    30. Report abnormal expression of insulin-like growth factor-II and its dynamic quantitative analysis at different stages of hepatocellular carcinoma development. PMID: 18693177
    31. A brain region- and cell type-specific responses to estrogen in rat brain, suggesting that Igf signaling may mediate the estrogen function in the hippocampus. PMID: 18946176
    32. nicotine exposure prevents the decrease of IGF-II processing in the maternal compartment; this may represent a compensatory mechanism allowing the mother to counteract the negative influence of nicotine on fetal growth and development PMID: 19494366
    33. IGF2 overexpression induced a clear protection against IL1B-induced beta-cell apoptosis. PMID: 19592596
    34. dual role of IGF-II in the control of IGF1R dependent cell apoptosis and involved activation of IGF2R signaling PMID: 19764351
    35. The placenta, the primary source of IGF-II synthesis in pregnancy, drives plasma volume expansion, and that IGF-II is among the regulatory factors of the gestational plasma volume increment. PMID: 19776249

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 数据库链接：