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  4. Rat Interferon α,IFN-α ELISA Kit

Rat Interferon α,IFN-α ELISA Kit

  • 中文名称：
    大鼠α干扰素(IFN-α)酶联免疫试剂盒
  • 货号：
    CSB-E08637r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat IFNA1 ELISA Kit was designed for the quantitative measurement of Rat IFNA1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

  • 别名：
    Ifna1 ELISA Kit; Interferon alpha-1 ELISA Kit
  • 缩写：
    IFN-α
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    31.25 pg/mL-2000 pg/mL
  • 灵敏度：
    7.81 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 85  
    Range % 81-91  
    1:2 Average % 92  
    Range % 85-97  
    1:4 Average % 99  
    Range % 94-104  
    1:8 Average % 103  
    Range % 99-108  
  • 回收率：
    The recovery of rat IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 92 87-96  
    EDTA plasma (n=4) 96 90-102  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    2000 2.087 2.099 2.093 1.916  
    1000 1.595 1.574 1.585 1.408  
    500 1.087 1.096 1.092 0.915  
    250 0.672 0.695 0.684 0.507  
    125 0.491 0.457 0.474 0.297  
    62.5 0.376 0.351 0.364 0.187  
    31.25 0.287 0.299 0.293 0.116  
    0 0.175 0.178 0.177 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Does this ELISA detect IFNalpha 1 or 2?

A:
Thanks for your inquiry. It is designed for IFN alpha 1.

靶点详情

  • 功能：
    Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Alpha/beta interferon family
  • 数据库链接：