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  4. Rat Interferon β,IFN-β/IFNB ELISA Kit

Rat Interferon β,IFN-β/IFNB ELISA Kit

  • 中文名称：
    大鼠β干扰素(IFN-β/IFNB)酶联免疫试剂盒
  • 货号：
    CSB-E04845r
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat IFNB1 ELISA Kit was designed for the quantitative measurement of Rat IFNB1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 别名：
    Ifnb1 ELISA Kit; IfnbInterferon beta ELISA Kit; IFN-beta ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %98
    Range %94-102
    1:2Average %104
    Range %98-110
    1:4Average %90
    Range %85-94
    1:8Average %93
    Range %86-98
  • 回收率：
    The recovery of pig LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-99
    EDTA plasma (n=4)8780-91
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.516 2.587 2.552 2.380
    5002.021 2.106 2.064 1.892
    2501.362 1.324 1.343 1.171
    1250.761 0.739 0.750 0.578
    62.50.503 0.534 0.519 0.347
    31.250.375 0.359 0.367 0.195
    15.60.276 0.295 0.286 0.114
    00.174 0.170 0.172
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

I will order IFN beta from you,
https://www.cusabio.com/Recombinant-Protein/Recombinant-Rat-Interferon-betaIfnb1-189.html
Could you guarantee I could measure that rat IFN beta with CSB-E04845r ELISA kit?
Is the two item compatible?

A:
Thanks for your inquiry.
The standard of this kit CSB-E04845r is NS0-derived recombinant protein.
The recombinant proteins with different expression systems will be different. Sorry we didn't validate the detailed differences before, so we can not 100% guarantee that the kit could measure the protein.We suggest you do a pretest first.

靶点详情

  • 功能：
    Has antiviral, antibacterial and anticancer activities.
  • 基因功能参考文献：
    1. results provided evidence that delivery of IL-18 and IFN-beta by BMSCs may be an excellent and promising approach to develop an effective treatment protocol for glioma therapy. PMID: 26252165
    2. IFN-gamma is able to activate rat intestinal mucous microvascular endothelial cells, thereby leading to upregulated expressions of IFN-alpha/beta. PMID: 20214528
    3. These findings validate IFN-beta as a therapeutic molecule for intraarticular gene therapy of arthritis. PMID: 16984225
    4. This study presents novel effects of IFN-beta in the central nervous system and begins to unravel the mechanism behind the known excitability changes in neurons. PMID: 19541371
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Alpha/beta interferon family
  • 数据库链接：