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Rat Interleukin 17,IL-17 ELISA KIT

  • 中文名称：
    大鼠白介素17(IL-17)酶联免疫试剂盒
  • 货号：
    CSB-E07451r
  • 规格：
    96T/48T
  • 价格：
    ￥3200/￥2500
  • 其他：

产品详情

  • 产品描述：
        IL17A（Interleukin-17A），又称为CTLAF-7 (Cytotoxic T-lymphocyte-associated factor 7)或者是IL-17，是一种细胞因子，由Th17淋巴细胞分泌，是一种免疫系统中的重要炎症介质。IL17A能够吸引和活化中性粒细胞、T淋巴细胞和单核细胞，同时还能刺激某些组织细胞产生其他炎症介质。人体内IL17A的含量与炎症反应及某些自身免疫性疾病的发生密切相关。
        j9九游会登录入口首页生物所提供的Rat Interleukin 17,IL-17 ELISA KIT属于ELISA检测试剂盒，采用双抗夹心法定量检测大鼠血清、血浆、组织匀浆样本中的IL17A，其灵敏度为23.43 pg/mL，检测范围为93.75 pg/mL-6000 pg/mL。
     
  • 别名：
    Il17a ELISA Kit; Ctla8 ELISA Kit; Il17Interleukin-17A ELISA Kit; IL-17 ELISA Kit; IL-17A ELISA Kit; Cytotoxic T-lymphocyte-associated antigen 8 ELISA Kit; CTLA-8 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    93.75 pg/mL-6000 pg/mL
  • 灵敏度：
    23.43 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 87  
    Range % 80-95  
    1:2 Average % 85  
    Range % 80-95  
    1:4 Average % 90  
    Range % 86-100  
    1:8 Average % 92  
    Range % 86-97  
  • 回收率：
    The recovery of rat IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 85-98  
    EDTA plasma (n=4) 93 88-101  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    6000 1.865 1.802 1.834 1.739  
    3000 1.418 1.376 1.397 1.302  
    1500 1.036 1.004 1.020 0.925  
    750 0.624 0.589 0.607 0.512  
    375 0.379 0.364 0.372 0.277  
    187.5 0.211 0.209 0.210 0.115  
    93.75 0.132 0.122 0.127 0.032  
    0 0.099 0.091 0.095 　  
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 最新研究进展：
    IL17A是一种介导炎症反应的细胞因子，在自身免疫疾病和肿瘤等多种疾病中发挥重要作用。最新的研究表明，IL17A在肺癌中的作用十分复杂，可能具有促进或抑制肿瘤发展的双重作用。此外，研究人员还发现，通过靶向IL17A和其受体可以增强肿瘤免疫应答和抑制肿瘤的生长和转移，为肿瘤治疗提供了新的方向。
  • 功能：
    Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. Signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation. Plays an important role in connecting T cell-mediated adaptive immunity and acute inflammatory response to destroy extracellular bacteria and fungi. As a signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites. In airway epithelium, mediates neutrophil chemotaxis via induction of CXCL1 and CXCL5 chemokines. In secondary lymphoid organs, contributes to germinal center formation by regulating the chemotactic response of B cells to CXCL12 and CXCL13, enhancing retention of B cells within the germinal centers, B cell somatic hypermutation rate and selection toward plasma cells. Effector cytokine of a subset of gamma-delta T cells that functions as part of an inflammatory circuit downstream IL1B, TLR2 and IL23A-IL12B to promote neutrophil recruitment for efficient bacterial clearance. Effector cytokine of innate immune cells including invariant natural killer cell (iNKT) and group 3 innate lymphoid cells that mediate initial neutrophilic inflammation. Involved in the maintenance of the integrity of epithelial barriers during homeostasis and pathogen infection. Upon acute injury, has a direct role in epithelial barrier formation by regulating OCLN localization and tight junction biogenesis. As part of the mucosal immune response induced by commensal bacteria, enhances host's ability to resist pathogenic bacterial and fungal infections by promoting neutrophil recruitment and antimicrobial peptides release. In synergy with IL17F, mediates the production of antimicrobial beta-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, limiting the entry of microbes through the epithelial barriers. Involved in antiviral host defense through various mechanisms.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    IL-17 family
  • 数据库链接：