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Rat Interleukin-34(IL34) ELISA kit

  • 中文名称:
    大鼠白介素-34(IL34)酶联免疫试剂盒
  • 货号:
    CSB-EL011657RA
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Rat IL34 ELISA Kit was designed for the quantitative measurement of Rat IL34 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

  • 别名:
    Il34Interleukin-34 ELISA Kit; IL-34 ELISA Kit
  • 缩写:
  • Uniprot No.:
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    25 pg/mL-1600 pg/mL
  • 灵敏度:
    6.25 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL34 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 89
    Range % 84-93
    1:2 Average % 107
    Range % 103-110
    1:4 Average % 85
    Range % 81-89
    1:8 Average % 89
    Range % 84-93
  • 回收率:
    The recovery of rat IL34 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 107 103-110
    EDTA plasma (n=4) 86 83-89
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1600 2.100 2.168 2.134 1.995
    800 1.654 1.604 1.629 1.490
    400 1.181 1.204 1.193 1.054
    200 0.765 0.778 0.772 0.633
    100 0.517 0.502 0.510 0.371
    50 0.346 0.351 0.349 0.210
    25 0.228 0.231 0.230 0.091
    0 0.140 0.138 0.139  
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 功能:
    Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1.
  • 基因功能参考文献:
    1. In a rat cardiac allograft model, IL-34 induced transplant tolerance associated with a total inhibition of alloantibody production. Treatment of rats with IL-34 promoted allograft tolerance mediated by induction of CD8+ and CD4+ Tregs. PMID: 26389674
  • 亚细胞定位:
    Secreted.
  • 蛋白家族:
    IL-34 family
  • 数据库链接: