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中文名称:大鼠白介素5(IL-5)酶联免疫试剂盒
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货号:CSB-E07435r
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规格:96T/48T
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价格:¥3200/¥2500
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其他:
产品详情
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产品描述:
This Rat IL5 ELISA Kit was designed for the quantitative measurement of Rat IL5 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .
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别名:Il5 ELISA Kit; Il-5 ELISA Kit; Interleukin-5 ELISA Kit; IL-5 ELISA Kit; B-cell growth factor II ELISA Kit; BCGF-II ELISA Kit; Cytotoxic T-lymphocyte inducer ELISA Kit; Eosinophil differentiation factor ELISA Kit; T-cell replacing factor ELISA Kit; TRF ELISA Kit
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缩写:
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Uniprot No.:
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种属:Rattus norvegicus (Rat)
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样本类型:serum, plasma, cell culture supernates, tissue homogenates
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检测范围:15.6 pg/mL-1000 pg/mL
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灵敏度:3.9 pg/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Immunology
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测定原理:quantitative
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测定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 85 Range % 82-91 1:2 Average % 92 Range % 83-99 1:4 Average % 101 Range % 93-107 1:8 Average % 96 Range % 90-104 -
回收率:
The recovery of rat IL-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 88 85-94 EDTA plasma (n=4) 94 90-100 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. pg/ml OD1 OD2 Average Corrected 1000 2.407 2.457 2.432 2.268 500 1.768 1.736 1.752 1.588 250 1.065 1.098 1.082 0.918 125 0.704 0.735 0.720 0.556 62.5 0.448 0.469 0.459 0.295 31.2 0.323 0.300 0.312 0.148 15.6 0.221 0.229 0.225 0.061 0 0.161 0.167 0.164 -
数据处理:
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货期:3-5 working days
引用文献
- Orally administered solasodine, a steroidal glycoalkaloid, suppresses ovalbumin-induced exaggerated Th2-immune response in rat model of bronchial asthma P Arora,Chemico-biological interactions,2022
- Lignosus rhinocerotis Cooke Ryvarden ameliorates airway inflammation, mucus hypersecretion and airway hyperresponsiveness in a murine model of asthma M Johnathan,Plos one,2021
- Potential anti-inflammatory and immunomodulatory effects of carvacrol against ovalbumin-induced asthma in rats El-Din Y M E,Life Sciences,2019
- In vitro-in vivo correlation of inhalable budesonide-loaded large porous particles for sustained treatment regimen of asthma Li J, et al,Acta Biomaterialia,2019
- Sustained therapeutic efficacy of budesonide-loaded chitosan swellable microparticles after lung delivery: Influence of in vitro release, treatment interval and dose Lan Zhang.et al,Journal of Controlled Release,2018
- Phytochemical profiles and inhibitory effects of Tiger Milk mushroom (Lignosus rhinocerus) extract on ovalbumin-induced airway inflammation in a rodent model of asthma. Johnathan M.et al,BMC Complement Altern Med.,2016
靶点详情
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功能:Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
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亚细胞定位:Secreted.
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蛋白家族:IL-5 family
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数据库链接:
STRING: 10116.ENSRNOP00000010729
UniGene: Rn.44227
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