Rat KI-67 ELISA kit

产品详情

文献引用 (1) 说明书下载

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

This Rat KI-67 ELISA Kit was designed for the quantitative measurement of Rat KI-67 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.04 ng/mL.
别名：

Nifk ELISA Kit; Mki67ipMKI67 FHA domain-interacting nucleolar phosphoprotein ELISA Kit; Nucleolar protein interacting with the FHA domain of pKI-67 ELISA Kit
缩写：

KI-67
Uniprot No.：

Q5RJM0
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.04 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat KI-67 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	83-90
1:2	Average %	93
	Range %	90-96
1:4	Average %	89
	Range %	85-93
1:8	Average %	100
	Range %	95-105

回收率：

The recovery of rat KI-67 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	92	88-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.