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  4. Rat Myoglobin,MYO/MB ELISA Kit

Rat Myoglobin,MYO/MB ELISA Kit

  • 中文名称：
    大鼠肌红蛋白(MYO/MB)酶联免疫试剂盒
  • 货号：
    CSB-E08049r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat Myoglobin(MB) ELISA Kit was designed for the quantitative measurement of Rat Myoglobin(MB) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL .

  • 别名：
    Mb ELISA Kit; Myoglobin ELISA Kit
  • 缩写：
    MB
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 ng/mL-1000 ng/mL
  • 灵敏度：
    3.9 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MYO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %94
    Range %90-100
    1:2Average %92
    Range %87-100
    1:4Average %97
    Range %90-105
    1:8Average %87
    Range %83-96
  • 回收率：
    The recovery of rat MYO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9688-99
    EDTA plasma (n=4)9591-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    10002.064 2.069 2.067 1.922
    5001.743 1.754 1.749 1.604
    2501.355 1.363 1.359 1.214
    1250.954 0.962 0.958 0.813
    62.50.718 0.728 0.723 0.578
    31.250.498 0.502 0.500 0.355
    15.60.337 0.343 0.340 0.195
    00.143 0.146 0.145 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
  • 基因功能参考文献：
    1. In rhabdomyolytic rats, the level of heme and free iron in cytoplasm and mitochondria of kidney cells is remarkably increased while inhibition of proteolysis results in further elevation of myoglobin content in the renal tissue. PMID: 27329933
    2. After reperfusion, there was no significant increase in dialysate myoglobin concentration. MDL-28170 did not affect dialysate myoglobin concentration during occlusion or after reperfusion. PMID: 24256333
    3. Amphetamine-induced serum myoglobin release is a potential biomarker and possibly a factor in AMPH-induced toxicity processes. PMID: 23608161
    4. bradykinin-induced NO does not interact significantly with cellular Mb to produce an NMR-detectable quantity of metMb in the perfused rat myocardium PMID: 14766675
    5. determination of myoglobin concentration and succinate dehydrogenase activity in serial sections indicate that myoglobin can lead to a substantial reduction (18-60%) of the extracellular oxygen tension required to prevent an anoxic core in muscle cells PMID: 15048578
    6. Mb-facilitated diffusion has a higher poise in skeletal muscle than in myocardium. PMID: 17038435
    7. Moreover, the similar Q10 for the DMb of solution versus cell Mb suggests that any temperature-dependent alteration of the postulated cell matrix does not significantly affect protein mobility. PMID: 17218454
    8. The nitrite reductase activity of deoxymyoglobin reduces nitrite approximately 36 times faster than deoxyhemoglobin because of its lower heme redox potential. PMID: 17293481
    9. intermittent hypobaric hypoxia decreased myocardial citrate synthase activity and myoglobin and decreased myocardial lactate dehydrogenase activity PMID: 19308875
    10. Data suggest that rhabdomyolysis-induced kidney damage involves direct interaction of myoglobin with mitochondria possibly resulting in iron ions release from myoglobin's heme, which promotes the peroxidation of mitochondrial membranes. PMID: 19545623

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  • 蛋白家族：
    Globin family
  • 数据库链接：