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  4. Rat NAD-dependent protein deacetylase sirtuin-1(SIRT1) ELISA kit

Rat NAD-dependent protein deacetylase sirtuin-1(SIRT1) ELISA kit

  • 中文名称：
    大鼠NAD依赖性蛋白脱乙酰酶sirtuin-1(SIRT1)酶联免疫试剂盒
  • 货号：
    CSB-EL021339RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat NAD-dependent protein deacetylase sirtuin-1(SIRT1) ELISA kit is the ideal tool for researchers working in the field of epigenetics and nuclear signaling. This quantitative sandwich assay is designed to detect and quantify the protein expression levels of NAD-dependent protein deacetylase sirtuin-1, also known as SIRT1, in serum, plasma, tissue homogenates, and cell lysates from Rattus norvegicus (Rat).

    With a detection range of 34.3 pg/mL-2200 pg/mL and a sensitivity of 8.5 pg/mL, this ELISA kit provides accurate and precise results for your research needs. The assay time is between 1-5 hours, and the required sample volume is only 50-100ul, making it a convenient and efficient tool for your laboratory.

    The detection wavelength of 450 nm ensures reliable and consistent results, and the kit comes with all the necessary components and reagents to perform the assay. With more than 3 citations, this kit has been validated and tested by other researchers, providing you with confidence in your results.

  • 别名：
    RP11-57G10.3, SIR2L1, SIR2alpha|sir2-like 1|sirtuin 1|sirtuin type 1
  • 缩写：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    34.3 pg/mL-2200 pg/mL
  • 灵敏度：
    8.5 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Epigenetics and Nuclear Signaling
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SIRT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %92
    Range %86-94
    1:2Average %102
    Range %97-105
    1:4Average %91
    Range %87-95
    1:8Average %98
    Range %92-102
  • 回收率：
    The recovery of rat SIRT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-98
    EDTA plasma (n=4)9995-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    22002.357 2.272 2.315 2.209
    11001.976 1.873 1.925 1.819
    5501.582 1.486 1.534 1.428
    2751.141 1.158 1.150 1.044
    137.50.761 0.757 0.759 0.653
    68.750.436 0.425 0.431 0.325
    34.30.262 0.266 0.264 0.158
    00.107 0.105 0.106
  • 数据处理：
  • 货期：
    3-5 working days

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