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  4. Rat Neurofilament light polypeptide(NEFL) ELISA kit

Rat Neurofilament light polypeptide(NEFL) ELISA kit

  • 中文名称：
    大鼠神经丝蛋白轻链多肽(NEFL)酶联免疫试剂盒
  • 货号：
    CSB-EL015688RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat NEFL ELISA Kit was designed for the quantitative measurement of Rat NEFL protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.

  • 别名：
    Nefl ELISA Kit; Nf68 ELISA Kit; Nfl ELISA Kit; Neurofilament light polypeptide ELISA Kit; NF-L ELISA Kit; 68 kDa neurofilament protein ELISA Kit; Neurofilament triplet L protein ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 pg/mL-500 pg/mL
  • 灵敏度：
    1.95 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat NEFL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %85-98
    1:2Average %107
    Range %97-111
    1:4Average %90
    Range %83-94
    1:8Average %97
    Range %91-102
  • 回收率：
    The recovery of rat NEFL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9082-94
    EDTA plasma (n=4)9991-104
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    5002.516 2.411 2.464 2.287
    2502.052 1.987 2.020 1.843
    1251.297 1.282 1.290 1.113
    62.50.725 0.784 0.755 0.578
    31.20.442 0.437 0.440 0.263
    15.60.338 0.330 0.334 0.157
    7.80.259 0.249 0.254 0.077
    00.178 0.176 0.177
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

I have a customer inquiring about CSB-EL015688RA. Could you let me know whether any preservatives are contained in each component? Could you also let me know whether it was contained recombinant protein in
this kit? If yes, Please let me know expression system of the recombinant protein.

A:
Thanks for your inquiry!
CSB-EL015688RA
Standard is NS0-derived recombinant protein.
Preservative is liquid proclin 300(1:3000).

靶点详情

  • 功能：
    Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
  • 基因功能参考文献：
    1. Furthermore, the effect of CaMKP on self-assembly of NFL was examined. Electron microscopy revealed that CaMKP markedly prevented NFL from forming large filamentous aggregates, suggesting that CaMKP-binding to NFL inhibits its filament association. These findings may provide new insights into a novel mechanism for regulating network formation of neurofilaments during neuronal differentiation. PMID: 27369073
    2. Data suggest that tetrahydropapaveroline (an endogenous catechol) causes oxidative stress resulting in astrocyte/neuronal cell death via generation of reactive oxygen species and modification/aggregation of NF-L (as in neurodegenerative diseases). PMID: 23228886
    3. In cultured cortical neurons, hamartin and tuberin co-localize with neurofilament light chain preferentially in the proximal to central growth cone region PMID: 12226091
    4. The amount of NF-L correlated with the morphological retinal and optic nerve damage in rats PMID: 15193534
    5. Increased neuritic degeneration was observed in neuronal cells overexpressing neurofilament light mutants. PMID: 15857389
    6. 68-kDa neurofilament light chain (NF-L) as identified as a binding protein of amino-terminal PH domain of PLC-gamma1. PMID: 16819285
    7. Rat pituitary cells from the proopiomelanocortin lineage nearly completely lack NF68 immunoreactivity. PMID: 16940710
    8. Data show that as a consequence of RhoA knockdown, the process which expresses neurofilament is markedly induced in C12 cells. PMID: 18556119

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  • 亚细胞定位：
    Cell projection, axon. Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Intermediate filament family
  • 组织特异性：
    Expressed in the dorsal root ganglion neurons (at protein level).
  • 数据库链接：