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Rat Neuron-specific enolase,NSE ELISA Kit

  • 中文名称:
    大鼠神经特异性烯醇化酶(NSE)酶联免疫试剂盒
  • 货号:
    CSB-E07963r
  • 规格:
    96T/48T
  • 价格:
    ¥3900/¥2500
  • 其他:

产品详情

  • 产品描述:

    This Rat ENO2 ELISA Kit was designed for the quantitative measurement of Rat ENO2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .

  • 别名:
    Eno2 ELISA Kit; Eno-2 ELISA Kit; Gamma-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 2 ELISA Kit; Neural enolase ELISA Kit; Neuron-specific enolase ELISA Kit; NSE ELISA Kit
  • 缩写:
  • Uniprot No.:
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.625 ng/mL-40 ng/mL
  • 灵敏度:
    0.156 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Metabolism
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %89
    Range %85-94
    1:2Average %100
    Range %97-104
    1:4Average %90
    Range %87-94
    1:8Average %100
    Range %95-103
  • 回收率:
    The recovery of rat NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9591-98
    EDTA plasma (n=4)9592-97
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    402.260 2.232 2.246 2.078
    201.454 1.486 1.470 1.302
    100.920 0.928 0.924 0.756
    50.654 0.663 0.659 0.491
    2.50.403 0.398 0.401 0.233
    1.250.322 0.314 0.318 0.150
    0.6250.266 0.257 0.262 0.094
    00.170 0.166 0.168  
  • 数据处理:
  • 货期:
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能:
    Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.
  • 亚细胞定位:
    Cytoplasm. Cell membrane.
  • 蛋白家族:
    Enolase family
  • 组织特异性:
    The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
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