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  4. Rat Pancreatic alpha-amylase(AMY2A) ELISA kit

Rat Pancreatic alpha-amylase(AMY2A) ELISA kit

  • 中文名称：
    大鼠胰淀粉酶α2A(AMY2A)酶联免疫试剂盒
  • 货号：
    CSB-EL001689RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat AMY2A ELISA Kit was designed for the quantitative measurement of Rat AMY2A protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 mIU/mL-200 mIU/mL and the sensitivity is 0.78 mIU/mL.

  • 缩写：
    AMY2A
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 mIU/mL-200 mIU/mL
  • 灵敏度：
    0.78 mIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat AMY2A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 100  
    Range % 95-104  
    1:2 Average % 88  
    Range % 84-93  
    1:4 Average % 90  
    Range % 85-94  
    1:8 Average % 99  
    Range % 94-105  
  • 回收率：
    The recovery of rat AMY2A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 92-101  
    EDTA plasma (n=4) 92 88-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml OD1 OD2 Average Corrected  
    200 2.627 2.574 2.601 2.497  
    100 1.985 1.852 1.919 1.815  
    50 1.104 1.126 1.115 1.011  
    25 0.628 0.609 0.619 0.515  
    12.5 0.360 0.381 0.371 0.267  
    6.25 0.259 0.247 0.253 0.149  
    3.12 0.181 0.174 0.178 0.074  
    0 0.103 0.105 0.104    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 基因功能参考文献：
    1. Comparative proteomic analysis revealed that amylase levels were decreased by twofold in pancreases from hyperlipidemic rats compared with control rats.Pancreatic amylase protein levels were also decreased in tissue from hyperlipidemic rats. PMID: 20981735
    2. analysis of how amylase secretion is proteoglycan-dependent in rat parotid acinar cells PMID: 17980695
  • 亚细胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Glycosyl hydrolase 13 family
  • 数据库链接：