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  4. Rat Paraoxonase 1 (PON1)ELISA Kit

Rat Paraoxonase 1 (PON1)ELISA Kit

  • 中文名称：
    大鼠对氧磷酶-1(PON1)酶联免疫试剂盒
  • 货号：
    CSB-E14340r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat PON1 ELISA Kit was designed for the quantitative measurement of Rat PON1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 μU/mL-1000 μU/mL and the sensitivity is 3.9 μU/mL.

  • 别名：
    Pon1 ELISA Kit; Pon ELISA Kit; Serum paraoxonase/arylesterase 1 ELISA Kit; PON 1 ELISA Kit; EC 3.1.1.2 ELISA Kit; EC 3.1.1.81 ELISA Kit; EC 3.1.8.1 ELISA Kit; Aromatic esterase 1 ELISA Kit; A-esterase 1 ELISA Kit; Serum aryldialkylphosphatase 1 ELISA Kit
  • 缩写：
    PON1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 μU/mL-1000 μU/mL
  • 灵敏度：
    3.9 μU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PON1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:200Average %91
    Range %86-95
    1:400Average %102
    Range %97-107
    1:800Average %91
    Range %85-97
    1:1600Average %97
    Range %91-103
  • 回收率：
    The recovery of rat PON1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)9790-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μU/mlOD1OD2AverageCorrected
    10002.733 2.799 2.766 2.616
    5002.274 2.220 2.247 2.097
    2501.718 1.708 1.713 1.563
    1251.103 1.123 1.113 0.963
    62.50.641 0.682 0.662 0.512
    31.20.351 0.362 0.357 0.207
    15.60.245 0.236 0.241 0.091
    00.150 0.149 0.150
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification.
  • 基因功能参考文献：
    1. Compared with the rats in the control group, the serum activity of xanthine oxidase was significantly higher and that of PON1 was significantly lower in the rats in the model nonalcoholic fatty liver disease group (P<0.01). PMID: 27959408
    2. Acai extract increased serum PON1 activity and reduced hepatic steatosis and hepatic injury in rats fed a high fat diet. PMID: 27642496
    3. investigated the changes in plasma PON1 activity, LDL oxidation, radical scavenging activity and lipid peroxidation in D-galactose-induced aging model and also compared the results with 24-month naturally aged rats PMID: 24214524
    4. Study documented, for the first time, the presence of PON3 in rat serum and, notably, found that the upregulation of PON3, rather than PON1, appears to be associated with physical training. PMID: 19091700
    5. High omega-3 PUFA and ethanol decreased liver PON1 mRNA expression. Betaine restored liver PON1 mRNA expressions in low and high omega-3 PUFA ETOH groups. PMID: 20028357
    6. increased N-homocysteinylation of plasma proteins in chronic hyperleptinemia caused by impairment of the animal's ability to hydrolyze homocysteine-thiolactone by PON1 PMID: 19887391
    7. increased PON1 level may have a function related to the well-known atherosclerosis resistance of rats PMID: 16052486
    8. caloric restriction reduced serum Paraoxonase 1 (PON1) activity and showed gender-related differences that are indicative of a different adaptive strategy of male and female rats when faced with a period of food restriction PMID: 16214326
    9. Result suggests that PON1 modulation is not an integral part of the main antioxidant mechanisms of caloric restriction. PMID: 16816326
    10. Serum PON1 activity increases within the first hours of fasting, representing a prompt adaptation designed to attenuate blood lipid peroxidation that cannot be sustained when fasting is prolonged PMID: 17146679
    11. Delivery of the PON-1 gene may be a possible therapeutic strategy for preventing atherosclerosis. PMID: 17460375
    12. Consumption of pistachio as 20% of daily caloric intake increased serum PON1 activity by 35%. PMID: 17464102
    13. Serum paraoxonase activities were increased in a dose-dependent manner in taurine-treated hypothyroid rats. PMID: 17645625
    14. Reduction in PON1 activity during Nippostrongylusbrasiliensis infection may be associated with inflammatory reactions. mounted against the parasites. PMID: 18458312
    15. Report gender related differences in paraoxonase 1 response to high-fat diet-induced oxidative stress. PMID: 18719679
    16. suggest a hepato-protective role for PON1 against inflammation, fibrosis and liver disease mediated by MCP-1. PMID: 19144177

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  • 亚细胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Paraoxonase family
  • 组织特异性：
    Plasma. Associated with HDL.
  • 数据库链接：