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  4. Rat Pentosidine ELISA Kit

Rat Pentosidine ELISA Kit

  • 中文名称：
    大鼠戊糖素(Pentosidine)酶联免疫试剂盒
  • 货号：
    CSB-E09416r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat Pentosidine ELISA Kit was designed for the quantitative measurement of Rat Pentosidine protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pmol/mL-1000 pmol/mL and the sensitivity is 3.9 pmol/mL .

  • 缩写：
    Pentosidine
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pmol/mL-1000 pmol/mL
  • 灵敏度：
    3.9 pmol/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Pentosidine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %84
    Range %80-89
    1:2Average %89
    Range %85-93
    1:4Average %96
    Range %92-100
    1:8Average %92
    Range %86-97
  • 回收率：
    The recovery of rat Pentosidine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10196-105
    EDTA plasma (n=4)9085-95
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pmol/mlOD1OD2AverageCorrected
    10002.065 2.051 2.058 1.966
    5001.361 1.292 1.327 1.235
    2500.745 0.697 0.721 0.629
    1250.389 0.412 0.401 0.309
    62.50.219 0.233 0.226 0.134
    31.20.189 0.201 0.195 0.103
    15.60.127 0.138 0.133 0.041
    00.094 0.089 0.092 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Regarding your ELISA kits CSB-E09416r, could you please advise on the following:
1) Are the standards natural material or recombinant? And if recombinant, what kind of cell or living material is it used for expression?
2) Do the standards include additive agents such as antiseptic or preservative? If they include, What is composition of additive agent?
3) Material name and concentration of Stop Solution.
4) Do these kits include hazardous substance such as azide, thimerosal, 2-ME, etc?

A:
Thanks for your inquiry.
CSB-E09416r Standard is purified Pentosidine polymer.
Preservative is porclin 300(1:3000).
Stop solution is 4N sulfuric acid；
These kits do not include hazardous substance such as azide, thimerosal, 2-ME, etc.
Pls let me know if you have any further questions. Thank you.