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  4. Rat Phosphotylinosital 3 kinase,PI3K ELISA Kit

Rat Phosphotylinosital 3 kinase,PI3K ELISA Kit

  • 中文名称：
    大鼠磷酸肌醇3激酶(PI3K)酶联免疫试剂盒
  • 货号：
    CSB-E08418r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat PI3K ELISA Kit was designed for the quantitative measurement of Rat PI3K protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

  • 缩写：
    PI3K
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PI3K in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 89-95  
    1:2 Average % 103  
    Range % 99-107  
    1:4 Average % 93  
    Range % 88-94  
    1:8 Average % 99  
    Range % 95-103  
  • 回收率：
    The recovery of rat PI3K spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 90-98  
    EDTA plasma (n=4) 96 91-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.860 2.778 2.819 2.679  
    500 2.231 2.222 2.227 2.087  
    250 1.379 1.404 1.392 1.252  
    125 0.748 0.761 0.755 0.615  
    62.5 0.464 0.448 0.456 0.316  
    31.2 0.307 0.302 0.305 0.165  
    15.6 0.218 0.211 0.215 0.075  
    0 0.141 0.139 0.140 　  
  • 数据处理：
  • 货期：
    3-5 working days

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