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  4. Rat Plasma kallikrein(KLKB1) ELISA kit

Rat Plasma kallikrein(KLKB1) ELISA kit

  • 中文名称：
    大鼠血浆激肽释放酶(KLKB1)酶联免疫试剂盒
  • 货号：
    CSB-EL012461RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat KLKB1 ELISA Kit was designed for the quantitative measurement of Rat KLKB1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 ng/mL-500 ng/mL and the sensitivity is 1.95 ng/mL .

  • 别名：
    Klkb1 ELISA Kit; Klk3 ELISA Kit; PkPlasma kallikrein ELISA Kit; EC 3.4.21.34 ELISA Kit; Fletcher factor ELISA Kit; Kininogenin ELISA Kit; Plasma prekallikrein) [Cleaved into: Plasma kallikrein heavy chain; Plasma kallikrein light chain] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 ng/mL-500 ng/mL
  • 灵敏度：
    1.95 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat KLKB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:200Average %91
    Range %86-95
    1:400Average %103
    Range %99-105
    1:800Average %91
    Range %85-97
    1:1600Average %96
    Range %91-99
  • 回收率：
    The recovery of rat KLKB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)9795-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    5002.648 2.524 2.586 2.493
    2502.335 2.287 2.311 2.218
    1251.894 1.804 1.849 1.756
    62.51.112 1.125 1.119 1.026
    31.20.647 0.600 0.624 0.531
    15.60.370 0.352 0.361 0.268
    7.80.241 0.222 0.232 0.139
    00.091 0.095 0.093 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family, Plasma kallikrein subfamily
  • 数据库链接：