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  4. Rat Protein S100-A1(S100A1) ELISA kit

Rat Protein S100-A1(S100A1) ELISA kit

  • 中文名称：
    大鼠S100-A1蛋白(S100A1)酶联免疫试剂盒
  • 货号：
    CSB-EL020622RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat S100A1 ELISA Kit was designed for the quantitative measurement of Rat S100A1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 ng/mL-400 ng/mL and the sensitivity is 1.56 ng/mL.

  • 别名：
    S100a1 ELISA Kit; Protein S100-A1 ELISA Kit; S-100 protein alpha chain ELISA Kit; S-100 protein subunit alpha ELISA Kit; S100 calcium-binding protein A1 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6.25 ng/mL-400 ng/mL
  • 灵敏度：
    1.56 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat S100A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 93
    Range % 89-97
    1:2 Average % 87
    Range % 82-91
    1:4 Average % 98
    Range % 94-102
    1:8 Average % 101
    Range % 95-107
  • 回收率：
    The recovery of rat S100A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 90-100
    EDTA plasma (n=4) 84 80-88
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    400 2.043 2.001 2.022 1.851
    200 1.295 1.327 1.311 1.140
    100 0.888 0.852 0.870 0.699
    50 0.608 0.640 0.624 0.453
    25 0.430 0.460 0.445 0.274
    12.5 0.346 0.329 0.338 0.167
    6.25 0.255 0.238 0.247 0.076
    0 0.168 0.174 0.171 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation. In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes. These changes allow interactions with specific target proteins and modulate their activity. Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase. Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole.
  • 基因功能参考文献：
    1. depletion of S100A1 was observed in ischemic cardiomyocytes, and S100A1 plasma concentration was also significantly higher than that of the sham-operated group PMID: 23683996
    2. S100A1 is involved in the maintenance of the genetic program that defines normal myocardial function and its downregulation is permissive for the induction of genes that underlie myocardial hypertrophy PMID: 12619862
    3. S100A1 and calmodulin compete for the same binding site on ryanodine receptor PMID: 18650434
    4. in sympathetic neurons exogenous S100A1 may lead to an increase of sympathetic output. PMID: 19657060
  • 亚细胞定位：
    Cytoplasm. Sarcoplasmic reticulum. Mitochondrion.
  • 蛋白家族：
    S-100 family
  • 组织特异性：
    Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.
  • 数据库链接：