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Rat Secretin (Sct) ELISA kit

  • 中文名称：
    大鼠促胰液素/胰泌素(Sct)酶联免疫试剂盒
  • 货号：
    CSB-E17968r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat Sct ELISA Kit was designed for the quantitative measurement of Rat Sct protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL .

  • 别名：
    Sct ELISA Kit; Secretin ELISA Kit
  • 缩写：
    Sct
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    3.12 pg/mL-200 pg/mL
  • 灵敏度：
    0.78 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Sct in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %81-97
    1:2Average %97
    Range %91-105
    1:4Average %95
    Range %89-99
    1:8Average %99
    Range %94-108
  • 回收率：
    The recovery of rat Sct spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9892-102
    EDTA plasma (n=4)9690-101
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.131 1.999 2.065 1.953
    1001.563 1.485 1.524 1.412
    500.945 0.967 0.956 0.844
    250.568 0.576 0.572 0.460
    12.50.369 0.357 0.363 0.251
    6.250.283 0.275 0.279 0.167
    3.120.178 0.181 0.180 0.068
    00.112 0.111 0.112 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Hormone involved in different processes, such as regulation of the pH of the duodenal content, food intake and water homeostasis. Exerts its biological effects by binding to secretin receptor (SCTR), a G-protein coupled receptor expressed in the basolateral domain of several cells. Acts as a key gastrointestinal hormone by regulating the pH of the duodenal content. Secreted by S cells of the duodenum in the crypts of Lieberkuehn and regulates the pH of the duodenum by (1) inhibiting the secretion of gastric acid from the parietal cells of the stomach and (2) stimulating the production of bicarbonate (NaHCO(3)) from the ductal cells of the pancreas. Production of bicarbonate is essential to neutralize the pH and ensure no damage is done to the small intestine by the gastric acid. In addition to regulating the pH of the duodenal content, plays a central role in diet induced thermogenesis: acts as a non-sympathetic brown fat (BAT) activator mediating prandial thermogenesis, which consequentially induces satiation. Mechanistically, secretin released by the gut after a meal binds to secretin receptor (SCTR) in brown adipocytes, activating brown fat thermogenesis by stimulating lipolysis, which is sensed in the brain and promotes satiation. Also able to stimulate lipolysis in white adipocytes. Also plays an important role in cellular osmoregulation: released into the systemic circulation in response to hyperosmolality and acts at different levels in the hypothalamus, pituitary and kidney to regulate water homeostasis. Also plays a role in the central nervous system, possibly by acting as a neuropeptide hormone: required for hippocampal synaptic function and neural progenitor cells maintenance.
  • 基因功能参考文献：
    1. propose that eyeblink conditioning (EBC)-induced secretin release from Purkinje cells modulates EBC acquisition by reducing surface expression of Kv1.2 at one or both of these sites PMID: 25403455
    2. Secretin decreases cell surface Kv1.2 alpha-subunit levels by modulating Kv1.2 alpha- subunit endocytic trafficking. PMID: 22764231
    3. Ursodeoxycholic acid is conjugated in order to promote secretin-stimulated hydrocholeresis in rats through Ae2, microtubules, intracellular Ca(2+), PKCalpha, PI3K, PKA, and MEK. PMID: 22194894
    4. Molecular basis of secretin docking to its intact receptor using multiple photolabile probes distributed throughout the pharmacophore. PMID: 21566140
    5. Data show that in secretin/secretin receptor binding, the alpha-helix in natural secretin was oriented approximately parallel to the amino-terminal alpha-helix of the receptor. PMID: 21388146
    6. Review. In the rat cerebellum, secretin functions as a retrograde messenger to facilitate GABA transmission, indicating that it can modulate motor and other functions. PMID: 12392059
    7. first immunohistochemical demonstration of secretin-immunoreactive elements in the brain stem and in primary sensory ganglia PMID: 12409229
    8. Secretin mRNA is present in gastric mucosa and the locally released secretin may exert a paracrine effect to inhibit acid secretion. PMID: 12609767
    9. role in activating chromogranin A transcription PMID: 12646581
    10. solitary nucleus neurons are activated by secretin as a consequence of activation of a non-selective cation conductance and support the emerging view that secretin can act as a neuropeptide within the CNS. PMID: 14715495
    11. COlitis in ganciclovir-treated Sec-simplex virus thymidine kinase mice arises from depletion of secretin gene-expressing lymphoid cells. PMID: 15337838
    12. secretin stimulates cholehepatic shunting of conjugated bile acids and is associated with increased cholangiocyte apical membrane ASBT PMID: 15834929
    13. Solid evidence for the role of secretin as a neuropeptide in the mammalian central nervous system. PMID: 15963647
    14. provide evidence of the endogenous release of secretin in the hypothalamus and the mechanisms underlying this release PMID: 16888165
    15. Atrial natriuretic factor may play a protective role by reducing secretin response to avoid overstimulation. PMID: 16973919
    16. propose that secretin works at multiple levels in the hypothalamus, pituitary, and kidney to regulate water homeostasis PMID: 19805236

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Glucagon family
  • 组织特异性：
    In the brain, expressed in the central amygdala, hippocampus, area postrema, nucleus of the tractus solitary and cerebellum. Expressed at higher level in the cerebellum.
  • 数据库链接：

    KEGG: rno:24769

    STRING: 10116.ENSRNOP00000024094

    UniGene: Rn.9919