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  4. Rat Stem cell factor/mast cell growth factor,SCF/MGF ELISA kit

Rat Stem cell factor/mast cell growth factor,SCF/MGF ELISA kit

  • 中文名称：
    大鼠干细胞因子/肥大细胞生长因子(SCF/MGF)酶联免疫试剂盒
  • 货号：
    CSB-E04720r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    The rat stem cell factor (SCF) Elisa kit is suitable for the quantitative measurement of rat SCFin serum, plasma, or tissue homogenates. This assay employs the sandwich enzyme immunoassay technique and enzyme-substrate chromogenic reaction. The color develops positively to the amount of rat SCF in samples. The color development is stopped and the intensity of the color is measured. This kit displays many advantages, including high sensitivity, strong specificity, good linearity, high precision and recovery, as well as lot-to-lot consistency.

    SCF, also called KITLG or MGF, is a major regulator of human and rodent mast cells (MC). SCF induces MC differentiation, as well as chemotaxis and mediator secretion in MC. It plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. SCF exerts its biological functions by binding to and activating the c-Kit receptor (CD117). SCF/CD117 signaling is key to the maintenance of the hematopoietic stem cells (HSCs) and progenitor cells in the bone marrow. SCF is abundantly expressed in the normal bone marrow and heart but is downregulated after myocardial infarction.

  • 别名：
    Kitlg; Kitl; Mgf; Kit ligand; Hematopoietic growth factor KL; Mast cell growth factor; MGF; Stem cell factor; SCF; c-Kit ligand
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6.25 pg/mL-400 pg/mL
  • 灵敏度：
    1.56 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SCF/MGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %100
    Range %95-106
    1:2Average %97
    Range %91-105
    1:4Average %88
    Range %84-92
    1:8Average %93
    Range %86-98
  • 回收率：
    The recovery of rat SCF/MGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)9288-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    4002.815 2.752 2.784 2.600
    2002.134 2.076 2.105 1.921
    1001.368 1.319 1.344 1.160
    500.920 0.934 0.927 0.743
    250.546 0.527 0.537 0.353
    12.50.403 0.421 0.412 0.228
    6.250.263 0.248 0.256 0.072
    00.183 0.185 0.184
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
  • 基因功能参考文献：
    1. the SCF/c-KIT system is aberrantly expressed in varicocele(VC) testis, which could be involved in male infertility caused by VC. PMID: 27707610
    2. It was concluded that the exposure of cadimium on newborn rats ovaries could inhibit follicle development. SCF/c-kit system might mediate follicle development damage caused by cadmium, which is associated with DNA hypomethylation of SCF/c-kit promoter region may be worthy of further study. PMID: 28801138
    3. up-regulation of the NPs/NPR-C and NPs/NPR-A, B/cGMP signaling pathways may be involved in depression-induced loss of gastric ICC by decreasing the production of mSCF PMID: 26862759
    4. Downregulation of either c-kit or CXCR4 in bone marrow stem cells decreased engraftment of circulating bone marrow stem cells and inhibited reparative effects of transmyocardial revascularization. PMID: 25420576
    5. A 100 nM dose of E2 unbalanced the SCF/c-kit system, with a crucial impact on germ cell survival and thus male fertility. PMID: 24825426
    6. SCF/c-kit signaling pathway is required for maintenance of interstitial cells of Cajal phenotype, survival, proliferation, and differentiation. PMID: 24228598
    7. In diabetic colonic dysmotility, deficiency of endogenous IGF-1 in colonic smooth muscle cells leads to reduction of SCF expression. PMID: 23745035
    8. The results of this study concluded that Electroacupuncture treatment up-regulates the positive cells and mRNA expression of SCF after cerebral ischemia/reperfusion. PMID: 22526758
    9. These results suggest that overload training inhibits the phagocytosis and the ROS generation of peritoneal macrophages, and that MGF produced by macrophages may play a key role in this process. PMID: 22592456
    10. activated mature cardiac mast cells are responsible, in a paracrine fashion, for the increase in mature mast cell density post aortocaval fistula by rapidly increasing SCF levels via the release of chymase. PMID: 22850284
    11. Stem cell factor gene transfer induces c-kit(+) stem/progenitor cell expansion in situ and cardiomyocyte proliferation, which may represent a new therapeutic strategy to reverse adverse remodeling after myocardial infarction. PMID: 22931954
    12. results indicate that KL-c-kit interaction plays a role in estrogenic regulation through oocyte-granulosa cell communication PMID: 22366471
    13. Expression of SCF+GM-CSF after hepatectomy can contribute to biliary remodeling (e.g., post-transplantation) by functional deregulation of the activity of key signaling intermediates involved in cell expansion and multipotent differentiation. PMID: 21932404
    14. SCF expression in gastric smooth muscle cells is stimulated by IGF-1 in both dose- and time-dependent manners through IGF-1R in which ERKMAPK signal transduction may play an important role. PMID: 21092510
    15. ability of ghrelin to modulate the expression of the SCF gene PMID: 15284210
    16. SCF released from astrocytes directs neural progenitor cell migration under hypoxic conditions. PMID: 17581219
    17. SCF and its receptor may play a role in the remodelling process of serum nephrotoxic nephritis. PMID: 18087173
    18. Stem cell factor/c-kit signaling mediated cardiac stem cell migration via activation of p38 MAPK. PMID: 18087667
    19. 4-vinylcyclohexene diepoxide compromises KIT/KITL signaling, which is critical for follicular survival in primordial and primary follicles. PMID: 18448842
    20. fetal exposures to DEHP have effects on FLC number, distribution, and most importantly, steroidogenic capacity and abnormal expressions of IGF1, KITL, and LIF genes may contribute to the reproductive toxicity of phthalates PMID: 18469139
    21. Follicle stimulating hormone has a protective effect on germ cells after unilateral testicular torsion, which is reflected by increased SCF expression. PMID: 18544282
    22. postsynaptic RapGAP SPAR is regulated by Polo-like kinase 2 and the SCFbeta-TRCP ubiquitin ligase in hippocampal neurons PMID: 18723513
    23. Upregulation of stem cell factor and c-kit expression occured after spinal cord injury. Stem cell factor could be useful as an agent for treatment of spinal cord injury. PMID: 19182706
    24. mRNA upregulated up to 20-fold in intrahepatic cholangiocarcinoma PMID: 19255573

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  • 亚细胞定位：
    [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Single-pass type I membrane protein. Cell projection, lamellipodium. Cell projection, filopodium.; [Soluble KIT ligand]: Secreted.
  • 蛋白家族：
    SCF family
  • 数据库链接：