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  4. Rat Sulfotransferase Family 1E Member 1(SULT1E1) ELISA Kit

Rat Sulfotransferase Family 1E Member 1(SULT1E1) ELISA Kit

  • 中文名称：
    大鼠雌激素硫酸转移酶(SULT1E1)酶联免疫试剂盒
  • 货号：
    CSB-E13472r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat SULT1E1 ELISA Kit was designed for the quantitative measurement of Rat SULT1E1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

  • 别名：
    Sult1e1 ELISA Kit; Ste ELISA Kit; Ste1 ELISA Kit; Sulfotransferase 1E1 ELISA Kit; ST1E1 ELISA Kit; EC 2.8.2.4 ELISA Kit; Estrogen sulfotransferase ELISA Kit; isoform 1 ELISA Kit; EST-1 ELISA Kit; Estrone sulfotransferase ELISA Kit; Sulfotransferase ELISA Kit; estrogen-preferring ELISA Kit
  • 缩写：
    SULT1E1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SULT1E1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %90
    Range %86-93
    1:2Average %108
    Range %99-111
    1:4Average %92
    Range %87-97
    1:8Average %99
    Range %92-103
  • 回收率：
    The recovery of rat SULT1E1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9487-97
    EDTA plasma (n=4)9990-102
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    102.488 2.377 2.433 2.300
    52.075 2.045 2.060 1.927
    2.51.412 1.389 1.401 1.268
    1.250.916 0.898 0.907 0.774
    0.6250.574 0.564 0.569 0.436
    0.3120.353 0.367 0.360 0.227
    0.1560.267 0.260 0.264 0.131
    00.134 0.132 0.133
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. Is a key enzyme in estrogen homeostasis, the sulfation of estrogens leads to their inactivation. Also sulfates dehydroepiandrosterone (DHEA), pregnenolone, (24S)-hydroxycholesterol and xenobiotic compounds like ethinylestradiol, equalenin, diethyl stilbesterol and 1-naphthol at significantly lower efficiency. Does not sulfonate cortisol, testosterone and dopamine.
  • 亚细胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    Sulfotransferase 1 family
  • 数据库链接：

    UniGene: Rn.32104