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  4. Rat Ubiquitin carboxyl-terminal hydrolase isozyme L1(UCHL1) ELISA kit

Rat Ubiquitin carboxyl-terminal hydrolase isozyme L1(UCHL1) ELISA kit

  • 中文名称：
    大鼠泛素羧基末端水解酶L1(UCHL1)酶联免疫试剂盒
  • 货号：
    CSB-EL025541RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) ELISA Kit, a highly sensitive and specific assay for the quantitative detection of UCHL1 in serum, plasma, tissue homogenates, and cell lysates from the Rat species.

    UCHL1,plays an important role in ubiquitin-proteasome system, is a protein that plays a crucial role in the regulation of cellular processes such as protein degradation and cell signaling. Dysregulation of UCHL1 has been implicated in various diseases, including Parkinson's disease and cancer. Our UCHL1 ELISA Kit offers a reliable and efficient means of measuring UCHL1 levels in biological samples, with a detection range of 0.78 ng/mL to 50 ng/mL and a sensitivity of 0.195 ng/mL.

    The assay principle of our UCHL1 ELISA Kit is based on the sandwich method. During the assay process, UCHL1 in the sample is sandwiched between two UCHL1-specific antibodies, which are coated on the plate. The bound UCHL1 is then detected using a detection antibody conjugated with HRP and a chromogenic substrate, producing a colorimetric signal that is directly proportional to the UCHL1 concentration.

    This UCHL1 ELISA Kit offers a quick assay time of 1-5 hours, with a sample volume of 50-100ul and a detection wavelength of 450 nm. It is suitable for use in cell biology research and has been optimized for use with Rat samples. Its quantitative assay principle, coupled with its wide detection range and high sensitivity, make it an invaluable tool for researchers studying the physiological and pathological roles of UCHL1 in cellular processes.

  • 别名：
    Uchl1 ELISA Kit; Ubiquitin carboxyl-terminal hydrolase isozyme L1 ELISA Kit; UCH-L1 ELISA Kit; EC 3.4.19.12 ELISA Kit; Neuron cytoplasmic protein 9.5 ELISA Kit; PGP 9.5 ELISA Kit; PGP9.5 ELISA Kit; Ubiquitin thioesterase L1 ELISA Kit
  • 缩写：
    UCHL1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.78 ng/mL-50 ng/mL
  • 灵敏度：
    0.195 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cell Biology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat UCHL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %89
    Range %84-93
    1:2Average %101
    Range %98-106
    1:4Average %89
    Range %85-93
    1:8Average %104
    Range %100-108
  • 回收率：
    The recovery of rat UCHL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8580-90
    EDTA plasma (n=4)9693-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    502.196 2.174 2.185 2.050
    251.618 1.635 1.627 1.492
    12.51.071 1.098 1.085 0.950
    6.250.682 0.654 0.668 0.533
    3.120.450 0.436 0.443 0.308
    1.560.356 0.368 0.362 0.227
    0.780.251 0.265 0.258 0.123
    00.134 0.136 0.135
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • 基因功能参考文献：
    1. UCH-L1 can inhibit TNF-alpha-induced vascular smooth muscle cell migration, and this kind of effect may be partially due to its suppression role in NF-kappaB activation. PMID: 30305579
    2. investigated temporal profile of astrocytic and neuronal injury markers after TBI; different mechanisms underlie clearance of UCH-L1 and GFAP in CSF and serum PMID: 25763798
    3. Potential of UCHL1 as biomarker for destruction of pancreatic beta cells. PMID: 25638021
    4. UCH-L1 is differently processed in neurons compared with clonal cell lines and that farnesylation does not account for the membrane association in neurons. PMID: 25326379
    5. The proteases HtrA2/Omi and UCH-L1 regulate TNF-induced necroptosis. PMID: 24090154
    6. in AD, Abeta may down-regulate UCH-L1 in the AD brain, which in turn impairs BDNF/TrkB-mediated retrograde signaling, compromising synaptic plasticity and neuronal survival. PMID: 23599427
    7. Restoring UCH-L1 activity by transduction of primary neurons with a UCH-L1 fusion protein protected neurons, while inhibition of UCH-L1 exacerbates hypoxic injury. PMID: 20933087
    8. PGP9.5 messenger was decreased in nitrofen-exposed animals on E18 (esophagus) or E15 (small bowel), and increased on E21 in the esophagus and E18 in small bowel. PMID: 21069350
    9. Zinc deficiency could induce downregulation of Uch-L1 expression. PMID: 19267071
    10. analysis of a possible mechanistic link between UCH-L1 modification by cyclopentenone prostaglandins and the etiology of neurodegeneration PMID: 20231490
    11. Complete loss of UCH-L1 immunoreactivity in orofacial skin nerve fibers in dithranol-treated animals. PMID: 17011532
    12. UCH-L1 is widely expressed in neurons in all brain regions analyzed, including dopamine neurons of the midbrain. In the periphery, UCH-L1 expression remains restricted to neurons in all organs examined. PMID: 17599367
    13. UCHL1 may partially attenuate vascular remodeling through inhibition of NF-kappaB activity. PMID: 17690318
    14. results demonstrated that zinc modulated the expression of ubiquitin C-terminal hydrolase L1 (Uch-L1) and cAMP-responsive element-binding protein (CREB) at the protein and/or transcription levels in hippocampal neurons PMID: 18616865

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  • 亚细胞定位：
    Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor.
  • 蛋白家族：
    Peptidase C12 family
  • 数据库链接：