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Rat Vascular Endothelial cell Growth Factor D,VEGF-D ELISA kit

  • 中文名称:
    大鼠血管内皮细胞生长因子D(VEGF-D)酶联免疫试剂盒
  • 货号:
    CSB-E07352r
  • 规格:
    96T/48T
  • 价格:
    ¥3200/¥2500
  • 其他:

产品详情

  • 产品描述:

    The Rat Vascular Endothelial cell Growth Factor D (VEGF-D) ELISA kit is a powerful tool for researchers studying the cardiovascular system in rats. VEGF-D is a protein known for its role in promoting the growth and survival of blood vessels, making it an important target for cardiovascular research.

    Our ELISA kit utilizes a quantitative sandwich assay principle to measure the levels of VEGF-D in serum, plasma, and tissue homogenates with a detection range of 0.312 ng/mL to 20 ng/mL and a sensitivity of 0.078 ng/mL. With an assay time of only 1-5 hours and a sample volume of 50-100ul, this kit is both efficient and convenient for researchers.

    Whether you are investigating the mechanisms of angiogenesis and lymphatic regeneration or studying the effects of cardiovascular disease on the vasculature, this Rat Vascular Endothelial cell Growth Factor D (VEGF-D) ELISA kit is the perfect tool for your research needs.

  • 别名:
    Vegfd ELISA kit; FigfVascular endothelial growth factor D ELISA kit; VEGF-D ELISA kit; c-Fos-induced growth factor ELISA kit; FIGF ELISA kit
  • 缩写:
    FIGF
  • Uniprot No.:
  • 种属:
    Rattus norvegicus (Rat)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.312 ng/mL-20 ng/mL
  • 灵敏度:
    0.078 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Cardiovascular
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGF-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)
    1:1 Average % 87
    Range % 83-92
    1:2 Average % 101
    Range % 97-105
    1:4 Average % 91
    Range % 85-97
    1:8 Average % 97
    Range % 91-103
  • 回收率:
    The recovery of rat VEGF-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 86-95
    EDTA plasma (n=4) 98 94-102
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    20 1.932 1.837 1.885 1.738
    10 1.456 1.566 1.511 1.364
    5 1.088 1.042 1.065 0.918
    2.5 0.713 0.707 0.710 0.563
    1.25 0.492 0.481 0.487 0.340
    0.625 0.358 0.342 0.350 0.203
    0.312 0.272 0.278 0.275 0.128
    0 0.148 0.146 0.147  
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 功能:
    Growth factor active in angiogenesis, lymphangiogenesis and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in the formation of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-3 (Flt4) receptor.
  • 基因功能参考文献:
    1. Expression of VEGF-D and its receptor, VEGFR-3, are enhanced in various cell types in the infarcted heart. PMID: 22818386
    2. Adenovirus encoding vascular endothelial growth factor-D induces tissue-specific vascular patterns in vivo. PMID: 12036873
    3. Overexpression of VEGF-D is associated with Gliosarcomas PMID: 16533777
    4. VEGF-D expression may have a role in interleukin (IL)-6 expression and transcription factor AP-1 activation in pancreatic acinar cells. PMID: 17404025
  • 亚细胞定位:
    Secreted.
  • 蛋白家族:
    PDGF/VEGF growth factor family
  • 组织特异性:
    Highly expressed in the spleen, kidney, lung, tongue, ovary and mammary gland.
  • 数据库链接: