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  4. Rat Vasoactive Intestinal Peptide,VIP ELISA Kit

Rat Vasoactive Intestinal Peptide,VIP ELISA Kit

  • 中文名称：
    大鼠血管活性肠肽(VIP)酶联免疫试剂盒
  • 货号：
    CSB-E08355r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat Vasoactive Intestinal Peptide (VIP) ELISA Kit is a highly sensitive and reliable assay for the quantification of VIP peptide in serum, plasma, and tissue homogenates from Rattus norvegicus (Rat).

    VIP peptide,a neuropeptide that is widely distributed in the central and peripheral nervous systems ,is known to play a critical role in the regulation of various physiological processes, particularly in the nervous system. Our ELISA kit is designed to accurately measure the levels of VIP peptides in biological samples, making it an essential tool for researchers in the field of neuroscience.

    The detection range of the kit is 1.56 pg/mL-100 pg/mL, with a sensitivity of 0.39 pg/mL, ensuring precise and accurate quantification of VIP peptides in samples. The assay time is only 1-5 hours, and the sample volume required is minimal (50-100ul), making the kit highly efficient and suitable for high-throughput experiments.

    The VIP ELISA kit uses a sandwich assay principle, which involves the use of a VIP-specific antibody to capture the peptide of interest, followed by the detection of the captured VIP peptide using a second VIP-specific antibody. The detection wavelength is 450 nm, and the results are easily obtained using a microplate reader.

  • 缩写：
    VIP
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 pg/mL-100 pg/mL
  • 灵敏度：
    0.39 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat VIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %94
    Range %90-98
    1:2Average %97
    Range %93-101
    1:4Average %100
    Range %97-103
    1:8Average %86
    Range %82-92
  • 回收率：
    The recovery of rat VIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9490-98
    EDTA plasma (n=4)9086-94
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.384 2.506 2.445 2.324
    501.732 1.813 1.773 1.652
    251.112 1.100 1.106 0.985
    12.50.615 0.626 0.621 0.500
    6.250.386 0.397 0.392 0.271
    3.120.297 0.286 0.292 0.171
    1.560.196 0.193 0.195 0.074
    00.121 0.120 0.121 　
  • 数据处理：
  • 货期：
    3-5 working days

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