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  4. Rat diamine oxidase(DAO) ELISA Kit

Rat diamine oxidase(DAO) ELISA Kit

  • 中文名称：
    大鼠二胺氧化酶(DAO)酶联免疫试剂盒
  • 货号：
    CSB-E12634r
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat DAO ELISA Kit was designed for the quantitative measurement of Rat DAO protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 mIU/mL-50 mIU/mL and the sensitivity is 0.195 mIU/mL .

  • 别名：
    Dao ELISA Kit; Dao1D-amino-acid oxidase ELISA Kit; DAAO ELISA Kit; DAMOX ELISA Kit; DAO ELISA Kit; EC 1.4.3.3 ELISA Kit
  • 缩写：
    DAO
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.78 mIU/mL-50 mIU/mL
  • 灵敏度：
    0.195 mIU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat DAO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %85
    Range %80-91
    1:200Average %93
    Range %89-99
    1:400Average %87
    Range %82-95
    1:800Average %101
    Range %98-104
  • 回收率：
    The recovery of rat DAO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8581-92
    EDTA plasma (n=4)9085-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/ml. OD1OD2AverageCorrected
    502.634 2.672 2.653 2.459
    252.267 2.298 2.283 2.089
    12.51.706 1.765 1.736 1.542
    6.251.114 1.156 1.135 0.941
    3.120.623 0.672 0.648 0.454
    1.560.492 0.480 0.486 0.292
    0.780.307 0.322 0.315 0.121
    00.193 0.195 0.194 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.
  • 基因功能参考文献：
    1. Plasma DAO activity decreases in response to severe intestinal mucosal injury after 5-FU treatment. PMID: 24005798
    2. Its toxic effect on motor neurons can be mediated by expression in motor neurons and it promotes autophagy. PMID: 24138986
    3. The study showed that the operation of renal ischemia resulted in a 45.49 +/- 8.30% (n = 8) decrease in the DAAO-induced consumption of substrate, indicating a sharp decrease in renal DAAO activity following this acute renal injury. PMID: 21110210
    4. Familial amyotrophic lateral sclerosis is associated with a mutation in D-amino acid oxidase PMID: 20368421
    5. results suggest that an increase in DAO expression in parts of the brain is involved in aberrant D-amino acid metabolism, may be a potential therapeutic target for schizophrenia PMID: 19685198
    6. This result clearly revealed that DAO was indispensable for the process of chiral inversion of d-leucine. PMID: 15026304
    7. These results suggest that the TAA repeats are important for expression of the D-amino-acid oxidase gene. PMID: 18716858

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  • 亚细胞定位：
    Peroxisome.
  • 蛋白家族：
    DAMOX/DASOX family
  • 数据库链接：