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  4. Rat dipeptidyl peptldase Ⅳ,DPPⅣ ELISA Kit

Rat dipeptidyl peptldase Ⅳ,DPPⅣ ELISA Kit

  • 中文名称：
    大鼠二肽基肽酶Ⅳ(DPPⅣ)酶联免疫试剂盒
  • 货号：
    CSB-E08519r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This rat DPP4 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of rat DPP4 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the DPP4 levels in the sample and is measured at 450 nm through a microplate reader.

    DPP4, also called CD26, is a serine protease that is widely distributed in various organs and cells. Through regulating energy metabolism, inflammation, and immune function, DPP4 is involved in various physiological and pathological processes such as inflammation, energy metabolism, immune regulation, cell adhesion, and apoptosis. During the COVID-19 global pandemic, DPP4 was found to be an important marker that may play a significant role in disease progression. DPP4 is also a surface marker expressed in senescent cells. DPP4 has also been demonstrated to be associated with CD8+ T cell co-stimulation, B cell activation, macrophage polarization, and NK cell proliferation, playing an emerging role in modulating immune functions. Regarding its role in the immune system, DPP4 was shown to participate in the pathogenesis of autoimmune diseases like rheumatoid arthritis, inflammatory bowel disease as well as graft-versus-host disease (GVHD).

  • 别名：
    Dpp4; Cd26; Dipeptidyl peptidase 4; Bile canaliculus domain-specific membrane glycoprotein; Dipeptidyl peptidase IV; DPP IV; GP110 glycoprotein; T-cell activation antigen CD26; CD antigen CD26
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.78 ng/mL - 50 ng/mL
  • 灵敏度：
    0.195 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat DPPⅣin various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 94    
    Range % 91-97    
    1:2 Average % 86    
    Range % 83-90    
    1:4 Average % 101    
    Range % 97-105    
    1:8 Average % 88    
    Range % 85-93    
  • 回收率：
    The recovery of rat DPPⅣ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 106 103-109    
    EDTA plasma (n=4) 95 91-99    
                   
                   
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline.
  • 基因功能参考文献：
    1. Study demonstrates that gestational glucocorticoid (GC) exposure during late pregnancy induced impaired glucose homeostasis that is accompanied by increased endoglin levels, atherogenic dyslipidemia and pancreatic beta cell dysfunction, increased DPP-4 activity and altered foetal outcome. Therefore, GC-induced glucose deregulation and inflammation during late gestation is through endoglin-/DPP-4-dependent pathway. PMID: 29677638
    2. Proteome profile array screening further revealed that DPPIV decreases matrix metalloproteinase-9, a key downstream effector of ERK-AKT signaling pathways PMID: 27525674
    3. results suggest that long-term exposure to DPPIV inhibitors looks compatible with an overall balanced metabolism PMID: 28895067
    4. Bis-Pyrano Prenyl Isoflavone Improves Glucose Homeostasis by Inhibiting Dipeptidyl Peptidase-4 in Hyperglycemic Rats PMID: 27238050
    5. The study demonstrates that the DPP4 inhibitor has a neuroprotective effect against hyperthermia-induced seizures by reducing the astrocyte-mediated inflammatory response by an NF-kappaB-dependent pathway. These properties are of significant clinical value and help explain the increasing evidence that DPP4 is involved in the incidence and progression of febrile seizures. PMID: 28372289
    6. DPP-4 inhibition with linagliptin may represent a novel treatment for chronic kidney disease. PMID: 27083282
    7. DPP4 inhibition protects against ventricular arrhythmias by attenuating NGF-induced sympathetic innervation via cAMP/PKA/CREB-dependent antioxidant pathway in non-diabetic infarcted rats. PMID: 26399925
    8. Dipeptidyl peptidase-4 inhibition by gemigliptin exerts a preventative effect on the proliferation and migration of VSMCs via Nrf2. PMID: 26187356
    9. Differences in Expression of DPP4 in Steatotic Rat Liver Are Not Related to Differences in the Methylation of its Gene Promoter PMID: 26359413
    10. The inhibition of the renal DPP-4 activity induced by saxagliptin may contribute to ameliorating renal injury in hypertension-related renal injury. PMID: 25936515
    11. Inhibition of DPP-4 reduces T2DM-induced increase in post-MI acute mortality possibly by restoring the autophagic response through attenuation of Bcl-2-Beclin-1 interaction. PMID: 26259714
    12. DPP4 inhibitor vildagliptin attenuates oxidative stress and cardiac fibrosis and improving cardiac function in rats with chronic myocardial infarction. PMID: 25823534
    13. Findings indicate a positive correlation between reduced stress-responsiveness and increased central NPY, in DPP4mut rats. PMID: 25635612
    14. DPP-4 inhibitors reduced macrophage infiltration via glucagon-like peptide-1-dependent signaling in nephritis model and suggest that the control of inflammation by DPP-4 inhibitors is useful for the treatment of nondiabetic kidney disease models. PMID: 25656369
    15. bone marrow derived cells but not the kidney represent at least one source of sDPP4. And leukocyte or macrophage subpopulations could be potential candidates. PMID: 24874705
    16. DPP4 regulates the expression of the hemoglobin genes, and might play a role in the preservation of renal function. PMID: 25122001
    17. Maternal deprivation as a model for postnatal stress experience influences remarkably postnatal lung development of rats, which is significantly modulated by the DPP4-system. PMID: 24357522
    18. Long term deficiency of DPP4 activity improved cardiac performance against pressure overload in rat. PMID: 24416433
    19. a low level of DPPIV activity contributed to maintaining intestinal homeostasis PMID: 23832365
    20. Circulating DPPIV activity is associated with poorer cardiovascular outcomes in heart failure. PMID: 23894014
    21. High fat and high cholesterol diets increased DPP-IV expression in intestinal lymph. PMID: 23535306
    22. DPP-IV is a promising in situ marker of biliary functionality not only of normal but also of fatty rats. PMID: 23361237
    23. Long term loss of DPP4 activity increased the capability against ROS stress, which was more than GLP-1 dependent pathway PMID: 23359639
    24. DPP-4 deficiency restored ischemia (but not G-CSF)-induced stem cell mobilization and improved vascular recovery in diabetic animals PMID: 23184393
    25. These data suggest that GLP-1 analogs may serve as a novel therapeutic drug to alleviate obesity-induced liver injury by reducing bile acid synthesis and improving liver bile secretory function. PMID: 22918684
    26. Dipeptidyl peptidase IV regulates proliferation of preglomerular vascular smooth muscle and mesangial cells. PMID: 22802229
    27. novel evidence shows the regulatory roles of DPP4 in chronic heart failure. PMID: 23035207
    28. Seven days post artery occlusion, dipeptidyl peptidase IV immunoreactivity was found in the perikarya of surviving cortical neurons of the ipsilateral hemisphere. PMID: 22373413
    29. APN and DPPIV activity levels are related to the development of arthritis, being protective or inducer of the susceptibility PMID: 21982785
    30. This study demonstrates a reduction of anabolic effects on the energy homeostasis and a centrally enhanced NPY-expression in DPP4-deficient rats under high caloric food intake. DPP4-deficiency altered intracorporal fat distribution. PMID: 20887754
    31. plasma DPP4 activity changes in accordance with the progression of hyperinsulinemic obesity and pancreatic islet atrophy PMID: 21139073
    32. Monosodium glutamate and/or food deprivation decreased the activity of dipeptidyl peptidase 4 in the soluble and membrane fraction from the hypothalamus, and in the membrane fraction from the hippocampus. PMID: 20153005
    33. Reduced airway inflammation in CD26-deficient F344 rats appear to be mediated by differences in the recruitment and activity of Tregs. PMID: 20560982
    34. APM and DPPIV-DI are respectively related to the downregulation of somatostatin in food-deprived rats, and to the recovery of energy balance in monosodium glutamate obese rats during food deprivation PMID: 19876009
    35. These findings demonstrate a negative regulatory role of the bronchus-associated lymphatic tissue -specific expression of CD26 in T-cell adhesion during an asthma-like inflammation. PMID: 19501934
    36. The 6A3 epitope was stably exposed in both native and denatured rDPP IV. Data mapped the 6A3 epitope to a surface-exposed Thr331-dependent motif D329KTTLVWN, only 11 amino acids away from L311QWLRRI on the same plane as the fifth beta-propeller blade. PMID: 19804410
    37. Immunoreactive endomorphin 2 was generated extracellularly by a membrane-bound DPP-IV, which was switched to "synthase" mode by the hydrolase inhibitor Ile-Pro-Ile PMID: 19540879
    38. Clustered charged amino acids of human adenosine deaminase comprise a functional epitope for binding the adenosine deaminase complexing protein CD26/dipeptidyl peptidase IV. PMID: 11901152
    39. DPPIV deficiency results in improved glucose tolerance and ameliorated insulin resistance PMID: 12031691
    40. kinetics of this enzyme and insights into its dimerization and gelatinase activity PMID: 12675219
    41. An increase in this enzyme is associated with graft rejection. Inhibition of this enzyme prevents heart graft rejection. PMID: 12675233
    42. The three-dimensional structure of DPPIV as determined by cryo-TEM and single particle analysis. PMID: 12705886
    43. identification of DPPIV-binding sites in fibronectin and the effect of binding site peptides on DPPIV/poly-fibronectin adhesion and metastasis PMID: 12716896
    44. The behavioral repertoire and response to ethanol was characterized in dipeptidyl peptidase IV (DPPIV/CD26)-deficient, mutant Japanese, German, and wild-type-like F344 rat substrains. PMID: 14568317
    45. presence of a yet to be characterized signalling mechanism whereby DPP IV has access to c-Src-containing signalling platforms PMID: 16478473
    46. short-term DP IV inhibition does not eliminate the satiety actions of exogenously administered peptide YY at the doses tested. PMID: 16802131
    47. results extend earlier findings and illustrate the role of CD26/DPP IV in alloantigen-mediated immune responses PMID: 16962474
    48. The X-ray structure confirms that the binding mode of rat DPPIV is similar to the parent xanthines. PMID: 17010607
    49. Inhibition of intragraft DPP IV activity significantly reduced ischemia/reperfusion-associated pulmonary injury, allowing for successful transplantation after 18 hours of ischemia. PMID: 17175274
    50. Rats with a high fat diet significantly increased DPP-IV's expression and activity about 142-152% in the intestine and 153-240% in kidneys, but there was no change in the liver. PMID: 17583752

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  • 亚细胞定位：
    [Dipeptidyl peptidase 4 soluble form]: Secreted.; Cell membrane; Single-pass type II membrane protein. Apical cell membrane; Single-pass type II membrane protein. Cell projection, invadopodium membrane; Single-pass type II membrane protein. Cell projection, lamellipodium membrane; Single-pass type II membrane protein. Cell junction. Membrane raft.
  • 蛋白家族：
    Peptidase S9B family, DPPIV subfamily
  • 组织特异性：
    Expressed in bile ducts and other epithelial brush borders (small intestine, kidney, colon, pancreatic duct); acinar structures in salivary glands; endothelial structures and T cell areas in thymus, spleen and lymph node.
  • 数据库链接：