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  4. Rat glucagon-like peptide-1,GLP-1 ELISA Kit

Rat glucagon-like peptide-1,GLP-1 ELISA Kit

  • 中文名称：
    大鼠胰高血糖素样肽1(Glp-1)酶联免疫试剂盒
  • 货号：
    CSB-E08117r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat Glp1 ELISA Kit was designed for the quantitative measurement of Rat Glp1 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 pg/mL-40 pg/mL and the sensitivity is 0.156 pg/mL .

  • 别名：
    N/A
  • 缩写：
    Glp1
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.625 pg/mL-40 pg/mL
  • 灵敏度：
    0.156 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat GLP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %86
    Range %84-95
    1:200Average %95
    Range %91-100
    1:400Average %84
    Range %81-89
    1:800Average %92
    Range %86-98
  • 回收率：
    The recovery of rat GLP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9187-96
    EDTA plasma (n=4)10395-108
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    402.607 2.744 2.676 2.531
    202.303 2.451 2.377 2.232
    101.817 1.922 1.870 1.725
    51.133 1.191 1.162 1.017
    2.50.621 0.632 0.627 0.482
    1.250.344 0.355 0.350 0.205
    0.6250.234 0.248 0.241 0.096
    00.140 0.150 0.145 　
  • 数据处理：
  • 货期：
    3-5 working days

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