Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Rat growth differentiation factor 15(GDF15)ELISA Kit

Rat growth differentiation factor 15(GDF15)ELISA Kit

  • 中文名称：
    大鼠生长分化因子15(GDF15)酶联免疫试剂盒
  • 货号：
    CSB-E16317r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat GDF15 ELISA Kit was designed for the quantitative measurement of Rat GDF15 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .

  • 别名：
    Gdf15 ELISA Kit; SbfGrowth/differentiation factor 15 ELISA Kit; GDF-15 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 pg/mL-100 pg/mL
  • 灵敏度：
    0.39 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat GDF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:10Average %92
    Range %87-96
    1:20Average %83
    Range %80-86
    1:40Average %96
    Range %93-99
    1:80Average %102
    Range %99-105
  • 回收率：
    The recovery of rat GDF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8683-90
    EDTA plasma (n=4)9389-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2Average
    1000.127 0.129 0.128
    500.208 0.220 0.214
    250.334 0.324 0.329
    12.50.472 0.498 0.485
    6.250.713 0.735 0.724
    3.121.162 1.139 1.151
    1.561.542 1.567 1.555
    02.814 2.864 2.839
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Binds to its receptor, GFRAL, and activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which constitutes part of the 'emergency circuit' that shapes feeding responses to stressful conditions (Probable). On hepatocytes, inhibits growth hormone signaling.
  • 基因功能参考文献：
    1. These findings demonstrate the importance of the AP in the mediation of cancer-dependent anorexia and body weight loss and support a pathological role of MIC-1 as a tumour-derived factor mediating CACS, possibly via an AP-dependent action. PMID: 28025863
    2. Plasma GDF-15 was increased 3, 12 and 26, while plasma TGF-beta was increased 12 weeks after irradiation. PMID: 27566082
    3. the time course of the expression of growth differentiation factor15 (GDF15) in rat ischemic myocardium with increasing durations of reperfusion, and to elucidate its physiopathological role in the noreflow phenomenon, is reported. PMID: 26647773
    4. findings indicate that the attenuation of REV-ERBalpha leads to an upregulation of Gdf10 and Gdf15 in decidual cells PMID: 26811051
    5. GDF15 protects the renal interstitium and tubular compartment in experimental type 1 and 2 diabetes without affecting glomerular damage PMID: 23986522
    6. Up-regulation of EGR-1 and NAG-1, along with higher reactive oxygen species load may positively regulate the intrinsic pathway of apoptosis for the NSAID mediated chemoprevention of colorectal cancer. PMID: 23435960
    7. Gdf15 expression exhibits increases >10-fold at night in the rat pineal gland, reaching levels that are >15-fold higher than the median in other tissues. PMID: 19103603

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    TGF-beta family
  • 组织特异性：
    Detected in plasma (at protein level).
  • 数据库链接：