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  4. Rat heat shock protein 27,HSP-27 ELISA Kit

Rat heat shock protein 27,HSP-27 ELISA Kit

  • 中文名称：
    大鼠热休克蛋白27(HSP-27)酶联免疫试剂盒
  • 货号：
    CSB-E09240r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat HSPB1 ELISA Kit was designed for the quantitative measurement of Rat HSPB1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.78 ng/mL-50 ng/mL and the sensitivity is 0.195 ng/mL.

  • 别名：
    Hspb1 ELISA Kit; Hsp27 ELISA Kit; Heat shock protein beta-1 ELISA Kit; HspB1 ELISA Kit; Heat shock 27 kDa protein ELISA Kit; HSP 27 ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.78 ng/mL-50 ng/mL
  • 灵敏度：
    0.195 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays):CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat HSP-27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 98    
    Range % 93-102    
    1:2 Average % 105    
    Range % 100-113    
    1:4 Average % 91    
    Range % 85-96    
    1:8 Average % 94    
    Range % 90-98    
  • 回收率：
    The recovery of rat HSP-27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 89 85-93    
    EDTA plasma (n=4) 85 80-90    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    50 2.361 2.293 2.327 2.126    
    25 1.674 1.592 1.633 1.432    
    12.5 1.002 1.056 1.029 0.828    
    6.25 0.729 0.704 0.717 0.516    
    3.12 0.447 0.471 0.459 0.258    
    1.56 0.335 0.318 0.327 0.126    
    0.78 0.278 0.269 0.274 0.073    
    0 0.196 0.205 0.201 　    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.
  • 亚细胞定位：
    Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle.
  • 蛋白家族：
    Small heat shock protein (HSP20) family
  • 组织特异性：
    Expressed in a variety of tissues. High levels in lung, adrenal, xiphoid, adipose tissue, heart and striated and smooth muscle, lower levels in the CNS. Adult levels are much higher in the slow-twitch soleus muscle than in the fast-twitch rectus femoris a
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