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  4. Rat hydroxyproline,Hyp ELISA Kit

Rat hydroxyproline,Hyp ELISA Kit

  • 中文名称：
    大鼠羟脯氨酸(Hyp)酶联免疫试剂盒
  • 货号：
    CSB-E08838r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat Hyp ELISA Kit was designed for the quantitative measurement of Rat Hyp protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.078 ng/mL.

  • 缩写：
    Hyp
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Hyp in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:200 Average % 87  
    Range % 83-90  
    1:400 Average % 94  
    Range % 90-98  
    1:800 Average % 101  
    Range % 97-104  
    1:1600 Average % 96  
    Range % 93-99  
  • 回收率：
    The recovery of rat Hyp spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-98  
    EDTA plasma (n=4) 100 95-105  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    10 0.112 0.115 0.114    
    5 0.186 0.179 0.183    
    2.5 0.260 0.269 0.265    
    1.25 0.471 0.439 0.455    
    0.625 0.615 0.629 0.622    
    0.312 1.117 1.139 1.128    
    0.156 2.173 2.229 2.201    
    0 2.854 2.897 2.876    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

问答及客户评论

■ 常见问题解答
Q:

Please let us know if it can be used with bone extract sample.

A:
Thanks for your inquiry.
Usually we suggest you test the sample types mentioned in the kit manual. You can do a pretest first if you want to test bone extract sample.
Q:

We are interested in sourcing Human, mouse, rat hydroxyproline,Hyp ELISA Kit from CusaBio. What would be our purchasing price for it?
Also, can you kindly clarify why they are a species specific elisa kit while hydroxyproline is a small molecule?

A:
Thanks for your inquiry.
I will ask our distributor in your area to give you a quote by email.
Hydroxyproline is small molecule and it is the same in different species. There are 3 reasons why we have species specific elisa kits.
1. For different species, the sample matrix is different and the characteristics of antibody used in the kits are also different.
2. The methods of these 3 kits are different. Human kit and mouse kit are based on double antibody sandwich method.
When the kit was developed, it was designed to mimic the presence of hydroxyproline in vivo. It can detect polymerized form. (including the polymerized form with other proteins and self-polymerization form). For human kit, capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody; For mouse kit, capture antibody is rat monoclonal antibody and detection antibody is goat polyclonal antibody.
Rat kit is based on competitive method. The plate is coated with mouse monoclonal antibody. HRP conjugated HYP competes with the HYP in the sample. The kit can detect the endogenous total HYP, including free form and polymerized form.
3. The reason why different kits use different methods is based on a variety of factors. You can choose the appropriate kit to avoid matrix problems.
Pls let me know if you have any further questions. Thank you.