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  4. Rat interferon-inducible protein 10,IP-10 ELISA Kit

Rat interferon-inducible protein 10,IP-10 ELISA Kit

  • 中文名称：
    大鼠干扰素诱导蛋白10(IP-10/CXCL10)酶联免疫试剂盒
  • 货号：
    CSB-E08182r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat CXCL10 ELISA Kit was designed for the quantitative measurement of Rat CXCL10 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 781.25 pg/mL-50000 pg/mL and the sensitivity is 195 pg/mL .

  • 别名：
    Cxcl10 ELISA kit; Inp10 ELISA kit; Mob1 ELISA kit; Scyb10C-X-C motif chemokine 10 ELISA kit; 10 kDa interferon gamma-induced protein ELISA kit; Gamma-IP10 ELISA kit; IP-10 ELISA kit; Interferon-inducible protein 10 ELISA kit; Protein Mob-1 ELISA kit; Small-inducible cytokine B10 ELISA kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    781.25 pg/mL-50000 pg/mL
  • 灵敏度：
    195 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %88
    Range %84-92
    1:2Average %94
    Range %90-98
    1:4Average %99
    Range %95-104
    1:8Average %102
    Range %96-108
  • 回收率：
    The recovery of rat IP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8993-96
    EDTA plasma (n=4)10095-105
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    500002.556 2.581 2.569 2.437
    250001.748 1.769 1.759 1.627
    125001.002 1.085 1.044 0.912
    62500.663 0.699 0.681 0.549
    31250.396 0.387 0.392 0.260
    1562.50.284 0.271 0.278 0.146
    781.250.219 0.230 0.225 0.093
    00.131 0.133 0.132 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects. Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites. Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization. In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation. Activation of the CXCL10/CXCR3 axis plays also an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization.
  • 基因功能参考文献：
    1. These observations provide evidence for IP-10 in a regulatory role in cold ischemia-elicited acute allograft rejection and in PRINS lncRNA expression PMID: 29880386
    2. CXCL10 is a circulating inflammatory marker elevated in advanced heart failure. PMID: 27271043
    3. data support our hypothesis that increased circulating IFN-gamma and glomerular TNF-alpha induce synergistically the production and secretion of CXCL10 by podocytes, attracting activated macrophages into kidney tissue. PMID: 25561167
    4. CXCL10 is a regulator for an increase of opioid-containing macrophages and antinociceptive mediator in inflammatory pain and as a key chemokine regulated by electroacupuncture PMID: 24732949
    5. CXCL10 may be a necessary algogenic molecule, especially in the development of Cancer-induced bone pain PMID: 24337539
    6. CXCL10 is involved in astrocyte migration in amyloid-beta-stimulated hippocampal astrocytes. PMID: 23270423
    7. CXCL10 is a potential candidate for the reduction in myelination in cultures on TnC-astrocytes PMID: 21917786
    8. CXCL10 and CCL5 but not CXCL11 are involved in the pathogenesis of multiple sclerosis. PMID: 20720435
    9. upregulated in endometriosis tissues PMID: 20452033
    10. interferon-inducible protein 10 (IP-10) as ethanol-responsive gene in the fetal rat brain PMID: 12354640
    11. Antibodies to this chemokine increase IFNgamma in BALF of F344 rats following Sendai virus infection. PMID: 12581489
    12. CXCL10-induced elevations in intracellular Ca2+ are associated with an increase in neuronal firing and an alteration in the relationship between the evoked Ca2+ signal and activity of hippocampal neurons. PMID: 15465598
    13. IP-10/CXCR3 signaling is associated with the pathogenesis of experimental autoimmune myasthenia gravis in rats. PMID: 15843529
    14. Our study demonstrates a role of IP-10/CXCL10 on T cell recruitment in a rat model of renal endothelial microvascular injury PMID: 16382019
    15. this study shows that IP-10 contributes to the regulation of the expression of SD (slit-diaphram) components not only in the physiologic state but also in pathologic states. PMID: 16382022
    16. Inhibition of CXCR3/IP-10 signaling can be considered as a potential treatment modality for myasthenia gravis. PMID: 19232748

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine alpha (chemokine CxC) family
  • 组织特异性：
    In the central nervous system, CXCL10 is predominantly localized to activated neurons. Expressed in both microglia and astrocytes.
  • 数据库链接：