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  4. Rat lipolysaccharide binding protein,LBP ELISA Kit

Rat lipolysaccharide binding protein,LBP ELISA Kit

  • 中文名称：
    大鼠脂多糖结合蛋白(LBP)酶联免疫试剂盒
  • 货号：
    CSB-E11184r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat LBP ELISA Kit was designed for the quantitative measurement of Rat LBP protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 别名：
    LbpLipopolysaccharide-binding protein ELISA Kit; LBP ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:5Average %85
    Range %81-89
    1:10Average %94
    Range %91-98
    1:20Average %91
    Range %85-97
    1:40Average %86
    Range %82-89
  • 回收率：
    The recovery of rat LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10097-103
    EDTA plasma (n=4)9490-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.869 2.899 2.884 2.688
    20002.231 2.268 2.250 2.054
    10001.514 1.569 1.542 1.346
    5000.969 0.999 0.984 0.788
    2500.628 0.648 0.638 0.442
    1250.432 0.469 0.451 0.255
    62.50.297 0.278 0.288 0.092
    00.197 0.195 0.196
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Plays a role in the innate immune response. Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. Acts as an affinity enhancer for CD14, facilitating its association with LPS. Promotes the release of cytokines in response to bacterial lipopolysaccharide.
  • 亚细胞定位：
    Secreted. Cytoplasmic granule membrane.
  • 蛋白家族：
    BPI/LBP/Plunc superfamily, BPI/LBP family
  • 组织特异性：
    Detected in lung, liver, heart and kidney.
  • 数据库链接：