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  4. Rat lipoprotein-associated phospholipase A2,Lp-PLA2 ELISA Kit

Rat lipoprotein-associated phospholipase A2,Lp-PLA2 ELISA Kit

  • 中文名称：
    大鼠脂蛋白磷脂酶A2(Lp-PLA2)酶联免疫试剂盒
  • 货号：
    CSB-E08320r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat Lp-PLA2 ELISA Kit was designed for the quantitative measurement of Rat Lp-PLA2 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.78 IU/mL-50 IU/mL and the sensitivity is 0.195 IU/mL.

  • 缩写：
    Lp-PLA2
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.78 IU/mL-50 IU/mL
  • 灵敏度：
    0.195 IU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Lp-PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 92    
    Range % 88-96    
    1:2 Average % 105    
    Range % 100-110    
    1:4 Average % 86    
    Range % 82-90    
    1:8 Average % 93    
    Range % 89-98    
  • 回收率：
    The recovery of rat Lp-PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 90-100    
    EDTA plasma (n=4) 88 84-92    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    IU/ml OD1 OD2 Average Corrected    
    50 2.203 2.361 2.282 2.152    
    25 1.924 2.040 1.982 1.852    
    12.5 1.512 1.459 1.486 1.356    
    6.25 0.936 0.902 0.919 0.789    
    3.12 0.477 0.485 0.481 0.351    
    1.56 0.300 0.318 0.309 0.179    
    0.78 0.241 0.234 0.238 0.108    
    0 0.132 0.127 0.130 　    
  • 数据处理：
  • 货期：
    3-5 working days

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