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  4. Rat monoamine oxidase(MAO) ELISA Kit

Rat monoamine oxidase(MAO) ELISA Kit

  • 中文名称：
    大鼠单胺氧化酶(MAO)酶联免疫试剂盒
  • 货号：
    CSB-E15076r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat MAO ELISA Kit was designed for the quantitative measurement of Rat MAO protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 93.75 mU/mL-6000 mU/mL and the sensitivity is 23.43 mU/mL.

  • 别名：
    Maoa; Amine oxidase [flavin-containing] A; Monoamine oxidase type A; MAO-A
  • 缩写：
    MAO
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    93.75 mU/mL-6000 mU/mL
  • 灵敏度：
    23.43 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MAO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:5Average %92
    Range %88-96
    1:10Average %96
    Range %90-102
    1:20Average %102
    Range %98-104
    1:40Average %87
    Range %83-92
  • 回收率：
    The recovery of rat MAO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9285-99
    EDTA plasma (n=4)8982-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/mlOD1OD2AverageCorrected
    60002.633 2.866 2.750 2.557
    30001.787 1.881 1.834 1.641
    15001.123 1.188 1.156 0.963
    7500.689 0.705 0.697 0.504
    3750.502 0.525 0.514 0.321
    187.50.369 0.360 0.365 0.172
    93.750.245 0.236 0.241 0.048
    00.195 0.191 0.193
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

May I know the composition of stop solution that is used for the monoamine oxidase ELISA Kit?

A:
Thanks for your inquiry. The stop solution of this kit is 4N sulfuric acid.Pls let me know if you have any further questions. Thank you.

靶点详情

  • 功能：
    Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.
  • 亚细胞定位：
    Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side.
  • 蛋白家族：
    Flavin monoamine oxidase family
  • 数据库链接：