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  4. Rat myeloperoxidase-antineutrophil cytoplasmic antibody(MPO-ANCA) ELISA kit

Rat myeloperoxidase-antineutrophil cytoplasmic antibody(MPO-ANCA) ELISA kit

  • 中文名称：
    大鼠髓过氧化物酶特异性抗中性粒细胞胞质抗体(MPO-ANCA)酶联免疫试剂盒
  • 货号：
    CSB-E08675r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat MPO-ANCA ELISA Kit was designed for the quantitative measurement of Rat MPO-ANCA protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.625 U/mL-40 U/mL and the sensitivity is 0.156 U/mL .

  • 别名：
    N/A
  • 缩写：
    MPO-ANCA
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.625 U/mL-40 U/mL
  • 灵敏度：
    0.156 U/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat MPO-ANCA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %85
    Range %80-95
    1:2Average %97
    Range %91-105
    1:4Average %96
    Range %92-100
    1:8Average %93
    Range %85-98
  • 回收率：
    The recovery of rat MPO-ANCA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9489-97
    EDTA plasma (n=4)9184-96
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    U/mlOD1OD2AverageCorrected
    402.612 2.752 2.682 2.518
    202.013 2.076 2.045 1.881
    101.367 1.304 1.336 1.172
    50.802 0.837 0.820 0.656
    2.50.444 0.424 0.434 0.270
    1.250.351 0.359 0.355 0.191
    0.6250.241 0.225 0.233 0.069
    00.160 0.167 0.164 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

Can you please confirm the following
1) Standard - Is it a recombinant or not? If it's recombinant, please let us know the origin. ( for ex;E-coli, bculovirus).
2) Antibodies →　Please let us know whether the antibodies include NaN3, Thimerosal or 2-Me as preservative. If it have any preservatives, please give us the content(%)
3) Stop Solution →　Please let us know the constituent and concentration. We would like to confirm about the toxicity.

A:
Thanks for your inquiry.
CSB-E08675r
This kit is for quantitative determination of antibody.
Standard is crude extract from natural samples.
Stop solution is 4N sulfuric acid.
Preservative is proclin 300(1:3000).
The kit doesn't contain NaN3, Thimerosal or 2-Me.
Pls let me know if you have any further questions. Thank you.