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  4. Rat nuclear factor-κB p65,NF-κB p65 ELISA Kit

Rat nuclear factor-κB p65,NF-κB p65 ELISA Kit

  • 中文名称：
    大鼠核因子-κB亚基p65亲和肽(NF-κB p65)酶联免疫试剂盒
  • 货号：
    CSB-E08788r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat NFκB p65 ELISA Kit was designed for the quantitative measurement of Rat NFκB p65 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL .

  • 别名：
    NF-Kappa B,nuclear factor-kappa B
  • 缩写：
    NFκB p65
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.56 pg/mL-100 pg/mL
  • 灵敏度：
    0.39 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat NF-κB p65 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:100Average %89
    Range %86-92
    1:200Average %99
    Range %97-103
    1:400Average %89
    Range %85-93
    1:800Average %95
    Range %91-99
  • 回收率：
    The recovery of rat NF-κB p65 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9591-97
    EDTA plasma (n=4)9490-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1002.039 2.022 2.031 1.887
    501.334 1.326 1.330 1.186
    250.908 0.902 0.905 0.761
    12.50.566 0.557 0.562 0.418
    6.250.358 0.364 0.361 0.217
    3.120.291 0.281 0.286 0.142
    1.560.243 0.232 0.238 0.094
    00.145 0.143 0.144 　
  • 数据处理：
  • 货期：
    3-5 working days

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