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  4. Rat prealbumin,PA ELISA Kit

Rat prealbumin,PA ELISA Kit

  • 中文名称：
    大鼠前白蛋白(PA)酶联免疫试剂盒
  • 货号：
    CSB-E13251r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat prealbumin (PA) ELISA Kit was designed for the quantitative measurement of Rat prealbumin (PA) protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 375 ng/mL-6000 ng/mL and the sensitivity is 375 ng/mL.

  • 别名：
    Ttr ELISA Kit; Tt ELISA Kit; Transthyretin ELISA Kit; Prealbumin ELISA Kit; TBPA ELISA Kit
  • 缩写：
    PA
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    375 ng/mL-6000 ng/mL
  • 灵敏度：
    375 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:200 Average % 95  
    Range % 91-99  
    1:400 Average % 103  
    Range % 99-107  
    1:800 Average % 98  
    Range % 95-103  
    1:1600 Average % 93  
    Range % 87-97  
  • 回收率：
    The recovery of rat PA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 99 95-103  
    EDTA plasma (n=4) 89 82-97  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    6000 0.152 0.163 0.158    
    3000 0.288 0.300 0.294    
    1500 0.561 0.568 0.565    
    750 0.903 0.925 0.914    
    375 1.477 1.434 1.456    
    0 2.662 2.591 2.627    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
  • 基因功能参考文献：
    1. Data suggest that the mutated transthyretin (TTR) seems to have potential as biomarker for early detection of hepatocellular carcinoma (HCC). PMID: 28876464
    2. Serum transthyretin level dramatically decreased in animals displaying decompression sickness symptoms. PMID: 27068327
    3. We conclude that stress upregulates TTR expression in choroid plexus. PMID: 22371232
    4. Decreased trophoblast expression of retinol transport proteins suggest that nitrofen may interfere with the fetal retinol transport resulting in reduced pulmonary RBP and TTR levels and causing pulmonary hypoplasia in CDH. PMID: 22015466
    5. Data demonstrate that, in vitro and in vivo, transthyretin is up-regulated by progesterone, and suggest that this response is mediated through a progesterone receptor-mediated mechanism. PMID: 20535645
    6. Structural basis of negative cooperativity in transthyretin PMID: 11995997
    7. Complex of rat transthyretin with tetraiodothyroacetic acid refined at 2.1 and 1.8 A resolution PMID: 11995998
    8. Comparison of binding interactions of dibromoflavonoids with transthyretin PMID: 11995999
    9. Amyloid formation in rat transthyretin: effect of oxidative stress PMID: 12135814
    10. data show the LR1 gene is up-regulated following short interval successive partial hepatectomy; it may play an important role in liver regeneration PMID: 12508371
    11. Data suggest that monomeric transthyretin may represent an ischemia-specific CSF marker to indicate the sequential changes according to ischemic insults of the brain. PMID: 15469881
    12. Our study provides genetic, behavioural and molecular evidence that TTR is involved in the maintenance of normal cognitive processes during aging by acting on the retinoid signalling pathway. PMID: 17512093
    13. Ttr mRNA expression in dorsal root ganglia and cauda equina. PMID: 18406527
    14. Sex hormones may increase TTR synthesis by the liver, and therefore, may contribute to amyloid deposition. PMID: 18568387
    15. Results suggest that transthyretin is up-regulated by estradiol via a pathway involving estrogen receptors alpha and beta. PMID: 19130215
    16. The expression of transthyretin in brain of a transgenic mouse model of Alzheimer's disease is asymmetric and affected by age and sex. PMID: 19167467
    17. rat and murine TTR have a lower intrinsic beta-aggregation propensity and a similar native beta-structure stability compared with human TTR. PMID: 19602727
    18. Transthyretin is expressed in the pineal gland >30-fold higher than the median expression level in other tissues, at levels similar to those in the choroid plexus. PMID: 19103603

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Transthyretin family
  • 组织特异性：
    Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in the choroid plexus. Detected at lower levels in the liver.
  • 数据库链接：

    KEGG: rno:24856

    STRING: 10116.ENSRNOP00000022113

    UniGene: Rn.1404