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  4. Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit

Rat regulated on activation in normal T-cell expressed and secreted/C-C motif chemokine 5 (RANTES/CCL5) ELISA kit

  • 中文名称：
    大鼠正常T细胞表达和分泌因子/趋化因子5(RANTES/CCL5)酶联免疫试剂盒
  • 货号：
    CSB-E07398r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat Ccl5 ELISA Kit is a reliable and precise tool for the detection and quantification of C-C motif chemokine 5 (Ccl5) in rat serum, plasma, cell culture supernates, and tissue homogenates. Ccl5 is an important protein that is involved in a variety of immune responses, including inflammation and the recruitment of immune cells to sites of infection.

    This ELISA kit features a sensitive detection range of 0.156 ng/mL to 10 ng/mL, with a sensitivity of 0.039 ng/mL. The assay principle is based on the sandwich measurement technique, providing researchers with quantitative results in as little as 1-5 hours. The kit is optimized for use with a sample volume of 50-100ul, making it a good solution for researchers.

    The Rat Ccl5 ELISA Kit is an ideal choice for researchers investigating immunology, inflammation, and immune responses in rats. With its sensitive detection range, efficient assay time, and cost-effective design, this kit is a valuable addition to any researcher's toolkit.

  • 别名：
    Ccl5 ELISA Kit; Scya5C-C motif chemokine 5 ELISA Kit; SIS-delta ELISA Kit; Small-inducible cytokine A5 ELISA Kit; T-cell-specific protein RANTES ELISA Kit
  • 缩写：
    RANTES/CCL5
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat RANTES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 99
    Range % 94-105
    1:2 Average % 102
    Range % 97-107
    1:4 Average % 92
    Range % 88-96
    1:8 Average % 101
    Range % 97-106
  • 回收率：
    The recovery of rat RANTES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 87 83-92
    EDTA plasma (n=4) 92 86-98
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.684 2.799 2.742 2.633
    5 2.178 2.349 2.264 2.155
    2.5 1.444 1.489 1.467 1.358
    1.25 0.783 0.795 0.789 0.680
    0.625 0.376 0.392 0.384 0.275
    0.312 0.258 0.264 0.261 0.152
    0.156 0.201 0.208 0.205 0.096
    0 0.108 0.109 0.109 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 数据库链接：