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  4. Rat relaxin 1 (RLN1) ELISA kit

Rat relaxin 1 (RLN1) ELISA kit

  • 中文名称：
    大鼠松弛肽/松弛素1(RLN1)酶联免疫试剂盒
  • 货号：
    CSB-EL019749RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat RLN1 ELISA Kit was designed for the quantitative measurement of Rat RLN1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 2.4 pg/mL-150 pg/mL and the sensitivity is 0.6 pg/mL.

  • 别名：
    Rln1 ELISA Kit; Rln ELISA Kit; Prorelaxin 1 [Cleaved into: Relaxin B chain; Relaxin A chain] ELISA Kit
  • 缩写：
    RLN1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    2.4 pg/mL-150 pg/mL
  • 灵敏度：
    0.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat RLN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %95
    Range %90-99
    1:2Average %89
    Range %84-93
    1:4Average %92
    Range %88-96
    1:8Average %97
    Range %92-101
  • 回收率：
    The recovery of rat RLN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 105100-110
    EDTA plasma (n=4)8480-88
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    1502.643 2.487 2.565 2.439
    752.097 2.236 2.167 2.041
    37.51.468 1.551 1.510 1.384
    18.750.907 0.945 0.926 0.800
    9.40.583 0.617 0.600 0.474
    4.70.384 0.401 0.393 0.267
    2.40.235 0.252 0.244 0.118
    00.121 0.130 0.126
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Relaxin is an ovarian hormone that acts with estrogen to produce dilatation of the birth canal in many mammals.
  • 基因功能参考文献：
    1. Relaxin-1 mRNA in the hearts of diabetic rats was significantly increased and that relaxin-3 mRNA expression at different time points was decreased. In addition, significant down-regulation of the mRNA expression of RXFP1 and RXFP3 was observed at 4 weeks after Streptozocin. PMID: 28776188
    2. The digestive tract has been shown to express relaxin receptors; the hormone appears to exert site-specific effects acting at the neural or at the smooth muscle level, mainly by a nitric oxide-mediated mechanism. (Review) PMID: 28606038
    3. We have shown that relaxin stimulates Sertoli cell proliferation through the activation of MEK/ERK1/2 and PI3K/AKT pathways. PMID: 24640565
    4. The aim of the present study was to explore a role of relaxin at specific stages of spermatogenesis using a co-culture of rat Sertoli and germ cells. PMID: 24640566
    5. Raxin is a mediator of the refractoriness of the mesangial cells to angiotensin II during pregnancy. PMID: 23207895
    6. Data demonstrate a functional role for relaxin in mediating uterine artery compliance in pregnant rats, which may be necessary to maintain adequate uterine blood flow to the uterus and placenta. PMID: 22744867
    7. A novel G-quadruplex in the relaxin gene, is reported. PMID: 22363579
    8. Rln-1 was localized in the granulosa cells of the ovarian follicles, and immunoreactivity against Rln 1 was increased by orthodontic tooth movement in a time-dependent manner. PMID: 21410550
    9. relaxin regulates fibroblast proliferation, differentiation, and collagen deposition and may have therapeutic potential in diseased states characterized by cardiac fibrosis. PMID: 15155573
    10. role for relaxin in the systemic hemodynamic changes of pregnancy, as well as novel therapeutic potential for relaxin in modifying arterial stiffness and cardiac afterload. PMID: 15198972
    11. High glucose concentration could stimulate the expression of endogenous relaxin. PMID: 19542742

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 数据库链接：

    KEGG: rno:25616

    STRING: 10116.ENSRNOP00000021315

    UniGene: Rn.9830