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Rat sex hormone-binding globulin (SHBG) ELISA kit

  • 中文名称：
    大鼠性激素结合球蛋白(SHBG)酶联免疫试剂盒
  • 货号：
    CSB-E12118r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat sex hormone-binding globulin (SHBG) ELISA kit is a high-quality research tool for measuring SHBG levels in Rattus norvegicus (rat) samples. This ELISA kit is designed to accurately quantify SHBG levels in a range of sample types including serum, plasma, cell culture supernates, tissue homogenates, and cell lysates.

    The Rat SHBG ELISA kit has a detection range of 375 ng/mL-6000 ng/mL and a sensitivity of 375 ng/mL, making it a highly precise tool for researchers studying sex function,thyroid/liver function and signal transduction pathways. With an assay time of 1-5 hours and a sample volume requirement of 50-100 ul, this kit is both efficient and cost-effective.

    The assay principle of the Rat SHBG ELISA kit is quantitative and based on a competitive measurement. The kit utilizes a high-quality antibody specific to SHBG, ensuring accurate and reliable results. The detection wavelength is 450 nm, providing a clear and precise readout of results.

    This ELISA kit has been widely cited in research publications, with over 5 citations to date. Researchers across the globe trust the accuracy and precision of the Rat SHBG ELISA kit, making it a must-have tool for any lab studying sex function,thyroid/liver function and signal transduction pathways.

  • 别名：
    Shbg ELISA Kit; Sex hormone-binding globulin ELISA Kit; SHBG ELISA Kit; Sex steroid-binding protein ELISA Kit; SBP ELISA Kit; Testis-specific androgen-binding protein ELISA Kit; ABP ELISA Kit
  • 缩写：
    SHBG
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates
  • 检测范围：
    375 ng/mL-6000 ng/mL
  • 灵敏度：
    375 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SHBG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 92  
    Range % 88-96  
    1:2 Average % 88  
    Range % 85-90  
    1:4 Average % 100  
    Range % 97-103  
    1:8 Average % 83  
    Range % 80-86  
  • 回收率：
    The recovery of rat SHBG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 87 82-91  
    EDTA plasma (n=4) 95 89-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    6000 0.204 0.201 0.203    
    3000 0.336 0.341 0.339    
    1500 0.789 0.772 0.781    
    750 1.800 1.806 1.803    
    375 2.312 2.353 2.333    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.
  • 基因功能参考文献：
    1. SHBG may be of functional importance for rapid effects of olfactory steroids on limbic functions including the control of reproductive behaviours through pheromones PMID: 24681170
    2. Fluorine had no effect on the expression of androgen binding protein and inhibin B mRNA in testis sertoli cells of Sprague Dawley rats. PMID: 21033444
    3. Upstream stimulatory factors (USF1 and USF2) induce Nr5a1 and Shbg gene expression during the onset of rat Sertoli cell differentiation. PMID: 21734262
    4. Data show that the use of alternative SHBG first exons 1A and 1B differentially inhibits translation from the ATG situated in exon 2, which codes for methionine 30 of transcripts that begin with the exon 1 sequence. PMID: 21079794
    5. Results suggest that Sertoli cell-derived androgen-binding protein acts on spermatids by modifying the transition protein-1 mRNA level. PMID: 12573823
    6. A wave of apoptosis during the pre-pubertal period is required for normal spermatogenesis to develop, and our data indicate that this apoptotic wave may be regulated by ABP and/or androgens. PMID: 12831397
    7. ABP was found in neurons of the magnocellular hypothalamic nuclei, the preoptic region and the lateral hypothalamus. ABP varicosities occurred throughout the hypothalamus, the median eminence and the posterior pituitary. PMID: 14625054
    8. ABP expression is regulated in differentiated Sertoli cells by the bHLH and CREB family of transcription factors. PMID: 15112319
    9. Estrogen receptor beta was strongly immunolabeled in pachytene spermatocytes of rat androgen-binding protein transgenic mice and localized in tubular stages in which TUNEL labeling was maximal. PMID: 15215204
    10. There is a significantly higher level of the Shbg transcript in hypodactylous rats compared to SHR controls. PMID: 15222128
    11. Intrahypothalamic expression of ABP may be among the mechanisms necessary for rapid actions of steroids on hypothalamic neuroendocrine systems. PMID: 15927785

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  • 亚细胞定位：
    Secreted. Note=In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis.
  • 组织特异性：
    Isoform 2 is only expressed in the liver.
  • 数据库链接：