Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Rat von Willebrand Factor,vWF ELISA Kit

Rat von Willebrand Factor,vWF ELISA Kit

  • 中文名称：
    大鼠血管性血友病因子/瑞斯托霉素辅因子(VWF)酶联免疫试剂盒
  • 货号：
    CSB-E08438r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    The Rat von Willebrand Factor, vWF ELISA Kit is a highly sensitive and reliable assay for the quantification of von Willebrand factor in serum, plasma, and tissue homogenates from Rattus norvegicus (Rat).

    von Willebrand factor is an important protein involved in the formation of blood clots and is crucial in the prevention of excessive bleeding. Our ELISA kit is designed to accurately measure the levels of von Willebrand factor in biological samples, making it an essential tool for researchers in the field of cardiovascular research.

    The detection range of the kit is 0.312 ng/mL-20 ng/mL, with a sensitivity of 0.078 ng/mL, ensuring precise and accurate quantification of von Willebrand factor in samples. The assay time is only 1-5 hours, and the sample volume required is minimal (50-100ul), making the kit highly efficient and suitable for high-throughput experiments.

    The vWF ELISA kit uses a sandwich assay principle, which involves the use of a vWF-specific antibody to capture the protein of interest, followed by the detection of the captured von Willebrand factor using a second vWF-specific antibody. The detection wavelength is 450 nm, and the results are easily obtained using a microplate reader.

    This vWF ELISA kit has been extensively validated, with over 13 citations in scientific literature, demonstrating its reliability and accuracy in detecting von Willebrand factor in biological samples.

  • 别名：
    Vwf ELISA Kit; von Willebrand factor ELISA Kit; vWF ELISA Kit; Fragment ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:100 Average % 91  
    Range % 86-99  
    1:200 Average % 97  
    Range % 92-105  
    1:400 Average % 85  
    Range % 81-87  
    1:800 Average % 88  
    Range % 83-95  
  • 回收率：
    The recovery of rat vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 90 87-93  
    EDTA plasma (n=4) 95 90-100  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.300 2.488 2.394 2.257  
    10 2.011 2.102 2.057 1.920  
    5 1.446 1.560 1.503 1.366  
    2.5 0.903 0.900 0.902 0.765  
    1.25 0.628 0.586 0.607 0.470  
    0.625 0.350 0.364 0.357 0.220  
    0.312 0.242 0.258 0.250 0.113  
    0 0.134 0.140 0.137 　  
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

靶点详情

  • 功能：
    Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
  • 亚细胞定位：
    Secreted. Secreted, extracellular space, extracellular matrix.
  • 组织特异性：
    Plasma.
  • 数据库链接：