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  4. Rat Xanthine Oxidase(XO) ELISA Kit

Rat Xanthine Oxidase(XO) ELISA Kit

  • 中文名称：
    大鼠黄嘌呤氧化酶(XOD)酶联免疫试剂盒
  • 货号：
    CSB-E13614r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    Introducing the Rat Xanthine Oxidase (XO) ELISA Kit - a highly sensitive and specific tool for the quantitative detection of Xanthine dehydrogenase in samples from Rattus norvegicus (Rat).

    This kit is designed to measure Xanthine Oxidase (XO) levels in serum, plasma, and tissue homogenates with a detection range of 0.312 ng/mL to 20 ng/mL, and a sensitivity of 0.078 ng/mL. The assay principle is quantitative and uses a sandwich measurement method.

    The assay is quick and easy, with an assay time of 1-5 hours and a sample volume of 50-100ul. The detection wavelength is 450 nm. The kit has been cited in more than 6 research papers and is a trusted and widely used product in the scientific community. Choose the Rat Xanthine Oxidase (XO) ELISA Kit for your research needs and get reliable and accurate results.

  • 别名：
    Xdh ELISA Kit; Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase ELISA Kit; XD ELISA Kit; EC 1.17.1.4); Xanthine oxidase ELISA Kit; XO ELISA Kit; EC 1.17.3.2 ELISA Kit; Xanthine oxidoreductase ELISA Kit; XOR)] ELISA Kit
  • 缩写：
    XDH
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.312 ng/mL-20 ng/mL
  • 灵敏度：
    0.078 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Others
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat XOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 87  
    Range % 82-93  
    1:2 Average % 96  
    Range % 91-103  
    1:4 Average % 98  
    Range % 96-105  
    1:8 Average % 97  
    Range % 92-102  
  • 回收率：
    The recovery of rat XOD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 98 94-104  
    EDTA plasma (n=4) 104 96-108  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    20 2.711 2.610 2.661 2.544  
    10 2.208 2.107 2.158 2.041  
    5 1.507 1.486 1.497 1.380  
    2.5 0.909 0.898 0.904 0.787  
    1.25 0.467 0.456 0.462 0.345  
    0.625 0.280 0.291 0.286 0.169  
    0.312 0.185 0.188 0.187 0.070  
    0 0.116 0.117 0.117 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species.
  • 基因功能参考文献：
    1. S-allyl cysteine as potent anti-gout drug: Insight into the xanthine oxidase inhibition and anti-inflammatory activity. PMID: 30059711
    2. Abdominal paracentesis drainage protects rats against severe acute pancreatitis-associated lung injury by reducing the mobilization of intestinal xanthine dehydrogenase. PMID: 27585949
    3. Our results show that XOR is important to the cardiovascular responses to nitrite in 2K1C hypertension, and XOR inhibitors commonly used by patients may cancel this effect. This finding suggests that nitrite treatment may not be effective in patients being treated with XOR inhibitors PMID: 27078869
    4. Hepatocyte growth factor prevents XO activation following hypoxia/reoxygenation primarily by inhibiting the JAK2 signaling pathway and in turn, inhibiting the increase in cytosolic Calcium. PMID: 27573711
    5. Treatment with etaftorone decreased expression of inducible NOS and XOR in kidneys, whereas it increased the expression of endothelial NOS. PMID: 26663724
    6. Results suggest that exaggerated XO activity and resultant increase in oxidative stress contributes to hypertension and xanthine oxidase inhibition could treat treating hypertension and related endothelial dysfunction. PMID: 27198514
    7. Transitional cell tumors induced by xanthine oxidase inhibitor topiroxostat in rats were due to physical stimulation to transitional cells of xanthine crystals/calculi. PMID: 24448833
    8. metabolism of sodium metabisulfite in liver tissue increased XO activity, induced ER stress and caused caspase activation which was attenuated by ghrelin treatment PMID: 25486021
    9. We have constructed a variant of the liver enzyme that lacks the carboxy-terminal amino acids 1316-1331; it appears to assume an intermediate form, exhibiting a mixture of dehydrogenase and oxidase activities. PMID: 25817260
    10. Data suggest that, with aging, up-regulation of reactive oxygen species derived from XO down-regulates uric acid excretion via down-regulation of active homodimer breast cancer resistance protein (Bcrp1, uric acid efflux transporter) in ileal mucosa. PMID: 26119820
    11. Rat Xdh is a moonlighting protein that has two distinct catalytic activities as a dehydrogenase and an oxidase. PMID: 15878860
    12. Allopurinol improves left ventricular contractile function and efficiency possibly by diminishing the known XO-mediated ROS effects on myofilament Ca(2+) sensitivity. PMID: 24014679
    13. crystal structures of the urate complexes of the demolybdo-form of the D428A mutant of rat xanthine oxidoreductase and of the reduced bovine milk enzyme PMID: 21077683
    14. 6-mercaptopurine treatment resulted in increased xanthine oxidase (Xdh) expression in the placenta. PMID: 22688000
    15. The present study indicates that enhancement of XO activity could be implicated in acrylonitrile-induced gastric damage in rats. PMID: 22648241
    16. inhibition ameliorates sympathetic innervation and arrhythmias PMID: 21295134
    17. Report differential effects of xanthine oxidase inhibition and exercise on albumin concentration in rat tissues. PMID: 20555367
    18. Xanthine oxidase inhibition can offer protection from radiation-induced endothelial dysfunction and cardiovascular complications. PMID: 20167676
    19. xanthine oxidase and nitric oxide synthase have a role in cocaine-induced oxidative damage to skin PMID: 20399619
    20. Aluminium increases xanthine oxidase activity and disturbs antioxidant status in the rat. PMID: 11787993
    21. a pivotal role in cytokine-induced bone resorption PMID: 12653206
    22. Contractile claudication causes an increase in xanthine oxidase activity in muscles. Inhibition of these oxidant sources protects against oxidative stress, muscle damage, and edema. PMID: 12969896
    23. 17alpha- and 17beta-estradiol modulate the hypoxia-induced regulation of XDH/XO activity at a posttranscriptional level by a receptor-independent mechanism PMID: 14588143
    24. role for locally synthesized XOR in the development of hypertension-associated end-organ damage, but no major role in the development of hypertension. PMID: 15201549
    25. Major part of reactive oxygen species (ROS) generation in acute rejection was contributed by xanthine oxidoreductase(XOR). ROS are associated with and contribute to acute allograft rejection because inhibition of XOR alleviated rejection phenomena. PMID: 15201667
    26. analysis of cysteine disulfide bonds and crystal structure of a non-convertible rat liver xanthine dehydrogenase mutant PMID: 15878860
    27. RONS formation by xanthine oxidase prevents activation of important signalling pathways, predominantly the MAPK-NF-kappaB pathway PMID: 15932896
    28. Nitrite-derived nitric oxide by xanthine oxidoreductase in hypoxic and acidic environment induced by hepatic ischemia reperfusion injury(I/R) protects liver against I/R injury in vivo. PMID: 16109514
    29. results point to an important role for xanthine oxidase in the early stage of hyperlipidemia-associated renal injury, mediating macrophage infiltration by a putatively redox-dependent upregulation of MCP-1 and VCAM-1 PMID: 16407880
    30. increased free radical generation-associated increase in SBP in aging rats is xanthine oxidase but not NAD(P)H oxidase-dependent PMID: 17031261
    31. Study presents a mutant of rat XOR that displays mainly XO activity with a superoxide:hydrogen peroxide production ratio of about 6:1. PMID: 17301076
    32. the NADH oxidase activity of both xanthine dehydrogenase (XD) and xanthine oxidase (XO) forms of rat liver xanthine oxidoreductase (XOR) and the potential role of this mammalian enzyme as an O2*- source PMID: 17440754
    33. data provide the first evidence indicating that the JAK-STAT pathway is involved in hypoxia-mediated xanthine dehydrogenase/oxidase (XDH/XO) activation in lung microvascular endothelial cells PMID: 17920330
    34. Hypoxia-exposed pups had evidence of increased serum and lung XO activity, increased vascular XO-derived superoxide production, and vascular nitrotyrosine formation. PMID: 18083771
    35. Xanthine oxidase and mitochondria contribute to vascular superoxide anion generation in DOCA-salt hypertensive rats. PMID: 18487445
    36. Mechanisms underlying erythrocyte and endothelial nitrite reduction to nitric oxide in hypoxia: role for xanthine oxidoreductase and endothelial nitric oxide synthase. PMID: 18818408
    37. Xanthine oxidase contributes to mechanical ventilation-induced diaphragmatic oxidative stress and contractile dysfunction. PMID: 18974366

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  • 亚细胞定位：
    Peroxisome. Cytoplasm. Secreted.
  • 蛋白家族：
    Xanthine dehydrogenase family
  • 数据库链接：