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Rat α-enolase ELISA Kit

  • 中文名称：
    大鼠α烯醇化酶(α-enolase)酶联免疫试剂盒
  • 货号：
    CSB-E17439r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat ENO1 ELISA Kit was designed for the quantitative measurement of Rat ENO1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

  • 别名：
    Eno1 ELISA Kit; Eno-1Alpha-enolase ELISA Kit; EC 4.2.1.11 ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Enolase 1 ELISA Kit; Non-neural enolase ELISA Kit; NNE ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.625 ng/mL-40 ng/mL
  • 灵敏度：
    0.156 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat α-enolase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 93
    Range % 88-97
    1:2 Average % 102
    Range % 97-107
    1:4 Average % 101
    Range % 95-105
    1:8 Average % 94
    Range % 91-98
  • 回收率：
    The recovery of rat α-enolase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 105 99-108
    EDTA plasma (n=4) 87 84-92
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    40 2.191 2.275 2.233 2.111
    20 1.448 1.538 1.493 1.371
    10 0.921 0.963 0.942 0.820
    5 0.563 0.572 0.568 0.446
    2.5 0.334 0.341 0.338 0.216
    1.25 0.231 0.245 0.238 0.116
    0.625 0.173 0.179 0.176 0.054
    0 0.121 0.122 0.122 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
  • 基因功能参考文献：
    1. gamma-enolase may signal and correlate lung tissue damage well before obvious physical pathological tissue damage and might be a candidate diagnostic and/or prognostic marker. PMID: 29728894
    2. The present study aimed to investigate the change of alpha-enolase during cardiac hypertrophy and explore its role in this pathological process. PMID: 28910549
    3. alpha-enolase has a catalytically independent role in inducing cardiomyocyte apoptosis and mitochondrial dysfunction. PMID: 25446184
    4. this research suggests that the protective effect of Hsc70 on the cardiomyocytes against oxidative stress is partly associated with its interaction with alpha-enolase. PMID: 21958194
    5. alpha-Enolase binds to RNA PMID: 21621582
    6. The alpha-enolase hyper-phosphorylation in hypertrophied left ventricle was accompanied by decreased enzymatic activity. PMID: 18468517
    7. Data show that the expression of alpha-enolase in the left ventricle of the rats during normal and pathological cardiac development is different. PMID: 19450578
    8. enolase alpha was up-regulated in left ventricle of spontaneously hypertensive rats. PMID: 16485256

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  • 亚细胞定位：
    Cytoplasm. Cell membrane. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
  • 蛋白家族：
    Enolase family
  • 组织特异性：
    Expressed in flagella of epididymal sperm. The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma
  • 数据库链接：