Your Good Partner in Biology Research

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Rat α-glutathione S-transferases,α-GST ELISA Kit

Rat α-glutathione S-transferases,α-GST ELISA Kit

  • 中文名称：
    大鼠α谷胱甘肽S转移酶(α-GST)酶联免疫试剂盒
  • 货号：
    CSB-E08907r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Rat Gsta1 ELISA Kit was designed for the quantitative measurement of Rat Gsta1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 mU/mL-10 mU/mL and the sensitivity is 0.039 mU/mL .

  • 别名：
    Gsta1 ELISA Kit; Glutathione S-transferase alpha-1 ELISA Kit; EC 2.5.1.18 ELISA Kit; 13-hydroperoxyoctadecadienoate peroxidase ELISA Kit; EC 1.11.1.- ELISA Kit; Androst-5-ene-3,17-dione isomerase ELISA Kit; EC 5.3.3.- ELISA Kit; GST 1-1 ELISA Kit; GST 1a-1a ELISA Kit; GST A1-1 ELISA Kit; GST B ELISA Kit; Glutathione S-transferase Ya-1 ELISA Kit; GST Ya1 ELISA Kit; Ligandin ELISA Kit
  • 缩写：
    Gsta1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    0.156 mU/mL-10 mU/mL
  • 灵敏度：
    0.039 mU/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat α-GST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 90  
    Range % 84-96  
    1:2 Average % 99  
    Range % 92-105  
    1:4 Average % 104  
    Range % 100-107  
    1:8 Average % 94  
    Range % 89-99  
  • 回收率：
    The recovery of rat α-GST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 85 82-89  
    EDTA plasma (n=4) 97 92-105  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mU/ml OD1 OD2 Average Corrected  
    10 2.588 2.599 2.594 2.394  
    5 1.884 1.899 1.892 1.692  
    2.5 1.265 1.287 1.276 1.076  
    1.25 0.852 0.898 0.875 0.675  
    0.625 0.588 0.598 0.593 0.393  
    0.312 0.452 0.469 0.461 0.261  
    0.156 0.374 0.397 0.386 0.186  
    0 0.199 0.201 0.200 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis. Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid.
  • 基因功能参考文献：
    1. The most sensitive, noninvasive biomarkers of HCBD-induced renal toxicity in Hanover Wistar rats were urinary alpha-GST and KIM-1. PMID: 21905055
    2. Mechanism of negative regulation of rat glutathione S-transferase A2 by the cytokine interleukin 6. PMID: 11939905
    3. Oltipraz-induced GSTA2 gene expression is dependent upon PI3-kinase-mediated nuclear translocation and binding of C/EBP-beta to the GSTA2 gene promoter. PMID: 12509401
    4. Single mutations are introduced to evaluate the importance of amino acids at the subunit interface of GSTA1-1. Phe52 and Arg69 are found to be major determinants of dimer formation: a single mutation at either position substantially hinders dimerization. PMID: 15035604
    5. P.893: Ceramide inhibits C/EBP-beta or Nrf2 activation, which contributes to repression of GSTA2 gene transactivation. P. 897: "...ceramide inhibition of... transcription factors contributes to the repression of the GSTA2 gene transactivation." PMID: 15319326
    6. C/EBP alpha and beta are involved in glucocorticoid-dependent repression of GSTA2 gene expression PMID: 17786629

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    GST superfamily, Alpha family
  • 数据库链接：

    KEGG: rno:24422

    UniGene: Rn.144550