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  4. Rat α1-Acid glycoprotein(α1-AGP) ELISA Kit

Rat α1-Acid glycoprotein(α1-AGP) ELISA Kit

  • 中文名称：
    大鼠α1酸性糖蛋白(α1-AGP)酶联免疫试剂盒
  • 货号：
    CSB-E12730r
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 别名：
    Orm1Alpha-1-acid glycoprotein ELISA kit; Orosomucoid ELISA kit; OMD ELISA kit
  • 缩写：
    ORM1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, urine, cell culture supernates, tissue homogenates
  • 检测范围：
    3.125 ng/mL-800 ng/mL
  • 灵敏度：
    3.125 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat α1-AGP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1000 Average % 95
    Range % 85-105
    1:2000 Average % 89
    Range % 81-98
    1:4000 Average % 90
    Range % 82-97
    1:8000 Average % 95
    Range % 83-98
  • 回收率：
    The recovery of rat α1-AGP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 95 89-102
    EDTA plasma (n=4) 95 90-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average
    800 0.213 0.217 0.215
    200 0.459 0.453 0.456
    50 0.851 0.859 0.855
    12.5 1.226 1.246 1.236
    3.125 1.727 1.740 1.734
    0 1.935 1.958 1.947
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Functions as transport protein in the blood stream. Binds various ligands in the interior of its beta-barrel domain. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
  • 基因功能参考文献：
    1. fatigue upregulates the level of ORM1, which in turn functions as an anti-fatigue protein to enhance muscle endurance via the CCR5 pathway. PMID: 26740279
    2. context-dependent nature of ORM1 angioregulatory function is further demonstrated in the CAM assay where ORM1 stimulates developmental angiogenesis without exerting any inhibitory activity PMID: 22916107
    3. alpha-1 acid glycoprotein expression is increased by retinoids at the transcriptional level through two distinct DR1 retinoic acid responsive elements PMID: 15157739
    4. Surprisingly, AGP-over-expressing mice were not protected against renal ischemia-reperfusion injury. PMID: 15502707
    5. Orm1 gene expression highly induced in cardiomyocytes overexpressing mineralocorticoid receptor and treated with aldosterone. PMID: 17234708

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Calycin superfamily, Lipocalin family
  • 数据库链接：