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  4. Sheep Albumin (Alb)ELISA Kit

Sheep Albumin (Alb)ELISA Kit

  • 中文名称：
    绵羊白蛋白酶联免疫试剂盒
  • 货号：
    CSB-E15839Sh
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：
        白蛋白（Albumin）是由肝脏合成并在血液中广泛存在的蛋白质。它的分子量较大，结构稳定，具有多种生物学功能。主要功能是维持血液的渗透压和血容量，调节离子和营养物质的运输，并参与药物代谢和免疫调节。此外，白蛋白还有助于细胞和组织的修复和再生。由于其丰富的生物学功能和广泛的应用价值，白蛋白已成为医学和生物科学研究中不可或缺的重要组成部分。

        j9九游会登录入口首页生物所提供的Sheep Albumin (Alb)ELISA Kit属于ELISA检测试剂盒，采用竞争法定量检测绵羊血清、血浆、细胞培养上清,尿液、组织匀浆样本中的ALB，其灵敏度为5.5 μg/mL，检测范围为350 μg/mL-21.88 μg/mL。
  • 别名：
    ALBSerum albumin ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Ovis aries (Sheep)
  • 样本类型：
    serum, plasma, cell culture supernates,Urine, Tissue Homogenates
  • 检测范围：
    21.88 μg/ml-350 μg/ml.
  • 灵敏度：
    5.5 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<12%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<15%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   

  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep albumin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:100 Average % 92    
    Range % 97-101    
    1:200 Average % 95    
    Range % 88-105    
    1:400 Average % 95    
    Range % 90-100    
    1:800 Average % 95    
    Range % 92-97    
  • 回收率：
    The recovery of sheep albumin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 87-102    
    EDTA plasma (n=4) 97 93-105    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    μg/ml OD1 OD2 Average      
    350 0.162 0.166 0.164      
    175 0.281 0.274 0.278      
    87.5 0.436 0.445 0.441      
    43.75 0.655 0.667 0.661      
    21.88 0.923 0.907 0.915      
    0 2.052 1.996 2.024      
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 最新研究进展：
        白蛋白（ALB）是血液中最丰富的蛋白质之一，它不仅具有多种重要的生理功能，如维持血容量和血压、输送药物和其他生物分子等，而且还被广泛应用于临床诊断和治疗。
        最近的研究表明，ALB在多种疾病中具有重要作用。例如，在COVID-19疫情中，患者的ALB水平下降通常与严重程度呈正相关，因此ALB可能是预测疾病严重程度和预后的重要指标。
        此外，在肾病和癌症等疾病中，ALB水平也被证明与疾病的发生和进展密切相关。例如，ALB的下降可能导致肾脏损伤和慢性肾脏病的发展，并被用作评估肾脏健康的重要指标；同时，ALB的降低也被发现与癌症的发生和恶化有关，因此可能作为肿瘤预后评估的潜在生物标志物之一。
        此外，ALB水平还可以用于评估营养状况，其下降通常与营养不良相关。
        综上所述，ALB是一个多功能的蛋白质，在不同的疾病中发挥着重要的作用，有望成为评估患者健康状况和预测预后的重要指标。
  • 功能：
    Binds water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Major calcium and magnesium transporter in plasma, binds approximately 45% of circulating calcium and magnesium in plasma. Potentially has more than two calcium-binding sites and might additionally bind calcium in a non-specific manner. The shared binding site between zinc and calcium at residue Asp-272 suggests a crosstalk between zinc and calcium transport in the blood. The rank order of affinity is zinc > calcium > magnesium. Binds to the bacterial siderophore enterobactin and inhibits enterobactin-mediated iron uptake of E.coli from ferric transferrin, and may thereby limit the utilization of iron and growth of enteric bacteria such as E.coli. Does not prevent iron uptake by the bacterial siderophore aerobactin.
  • 基因功能参考文献：
    1. We have purified SSA from sheep kidneys by a simple and efficient two-step purification procedure. Further, we have studied urea-induced denaturation of SSA by monitoring changes in the difference absorption coefficient at 287nm intrinsic fluorescence emission intensity and mean residue ellipticity at 222nm at pH 7.4 and 25 degrees C PMID: 27180298
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    ALB/AFP/VDB family
  • 组织特异性：
    Plasma.
  • 数据库链接：

    KEGG: oas:443393

    UniGene: Oar.449