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Sheep Insulin-like growth factor II(IGF2) ELISA kit

  • 中文名称：
    羊胰岛素样生长因子II(IGF2)酶联免疫试剂盒
  • 货号：
    CSB-EL011088SH
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Sheep IGF2 ELISA Kit was designed for the quantitative measurement of Sheep IGF2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.

  • 别名：
    IGF2Insulin-like growth factor II ELISA Kit; IGF-II) [Cleaved into: Insulin-like growth factor II; Preptin] ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Ovis aries (Sheep)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 ng/mL-1000 ng/mL
  • 灵敏度：
    3.9 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of sheep IGF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 86-99  
    1:2 Average % 97  
    Range % 91-104  
    1:4 Average % 95  
    Range % 92-98  
    1:8 Average % 98  
    Range % 91-105  
  • 回收率：
    The recovery of sheep IGF2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-97  
    EDTA plasma (n=4) 96 90-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    1000 2.902 2.954 2.928 2.751  
    500 2.031 2.164 2.098 1.921  
    250 1.089 1.075 1.082 0.905  
    125 0.592 0.553 0.573 0.396  
    62.5 0.362 0.359 0.361 0.184  
    31.25 0.278 0.298 0.288 0.111  
    15.6 0.235 0.213 0.224 0.047  
    0 0.176 0.177 0.177 　  
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF2 is influenced by placental lactogen. Also involved in tissue differentiation. In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver. Acts as a ligand for integrin which is required for IGF2 signaling. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation. Inhibits myoblast differentiation and modulates metabolism via increasing the mitochondrial respiration rate.; Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
  • 基因功能参考文献：
    1. The mRNA expression levels of MSTN, IGF-1 and IGF-2 were significantly different in the same skeletal muscle under different dietary intake. PMID: 25078581
    2. Data show that both short- and long-term exposure to high-altitude conditions decreased luteal growth and IGF-I and IGF-II gene expression but increased HIF-1 alpha and VEGF immunoexpression. PMID: 23521851
    3. In PR fetal sheep, IGF-2, IGF-1R and IGF-2R mRNA expression was increased in the heart compared to controls. PMID: 21807611
    4. weight loss in both normal and overweight mothers during the periconceptional period results in epigenetic modification of IGF2 in the adrenal gland, adrenal overgrowth, and increased vulnerability to stress in offspring PMID: 20371620
    5. investigation whether the ovine H19-IGF2 and IGF2R loci are imprinted and analysis of their DNA methylation status in cloned lambs [IGF2, H19, & IGF2R] PMID: 14654216

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 数据库链接：

    KEGG: oas:443325

    UniGene: Oar.376